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goat anti-6 His FITC polyclonal antibody 6511

$496.00

Antibody summary

  • Goat polyclonal to 6 His FITC
  • Suitable for: IHC,ICC,IF
  • Isotype: Whole IgG
  • 100 µg
SKU: 6511parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

goat

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

6 His

available sizes

100 µg

Available product – goat anti-6 His FITC polyclonal antibody 6511

antibody
Tested applications
IHC,IHC,ICC/IF
Immunogen
6-His (HHHHHH) conjugated to KLH.
Size and concentration
100µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at 2-8°C.
Storage buffer
Phosphate Buffered Saline (PBS) containing 0.2%
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689
donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699
donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704
donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709
donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control
Goat polyclonal - Isotype Control
target relevance
Biotechnology
The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting. The versatility and simplicity of the 6x His tag have significantly advanced research efforts in protein expression, characterization, and functional analysis, allowing researchers to explore the structure and function of a wide range of proteins in diverse biological systems.

Data

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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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