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goat anti-6 His polyclonal antibody 7632

$426.00$2,415.00

Antibody summary

  • Rabbit polyclonal to 6 His
  • Suitable for: WB, ICC/IP, IP, ELISA
  • Isotype: IgG
  • 100 µg, 1 mg
SKU: 7632parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ELISA, ICC/IF, WB

reactivity

6 His

available sizes

1 mg, 100 µg

Available product – goat anti-6 His polyclonal antibody 7632

antibody
Tested applications
WB,ICC/IF,ELISA,IP
Recommended dilutions
Immunoblotting: use at a dilution of 1:1,000-1:20,000.

ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating, use at a dilution of 1:100- 1:500.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use at 1-4ug/mg lysate

These
Immunogen
6-His (HHHHHH) conjugated to KLH.
Size and concentration
100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at 2-8°C.
Storage buffer
PBS (PBS), pH 7.2, 0.09% NaN3.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Biotechnology
The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting. The versatility and simplicity of the 6x His tag have significantly advanced research efforts in protein expression, characterization, and functional analysis, allowing researchers to explore the structure and function of a wide range of proteins in diverse biological systems.

Data

benchmark-antibodies_anti-6_his_antibody_7632_1.jpg
Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-6-His antibody 7632 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
benchmark-antibodies_anti-6_his_antibody_7632_1.jpg
Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-6-His antibody 7632 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
benchmark-antibodies_anti-6_his_antibody_7632_2.jpg
Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, rabbit anti-6-His antibody 7632 used for WB at 0.04 µg /ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg /ml) and chemiluminescence with an exposure time of 10 seconds.
benchmark-antibodies_anti-6_his_antibody_7632_2.jpg
Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, rabbit anti-6-His antibody 7632 used for WB at 0.04 µg /ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg /ml) and chemiluminescence with an exposure time of 10 seconds.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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