| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| reactivity | 6 His |
| available sizes | 100 µg |
goat anti-6 His polyclonal antibody 7632
$426.00
Antibody summary
- Rabbit polyclonal to 6 His
- Suitable for: WB, ICC/IP, IP, ELISA
- Isotype: IgG
- 100 µg
goat anti-6 His polyclonal antibody 7632
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:20,000. ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating, use at a dilution of 1:100- 1:500. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use at 1-4ug/mg lysate These |
| Immunogen 6-His (HHHHHH) conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS (PBS), pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| 6X His tag |
| Protein names Hexahistidine tag |
| Alternative names 6xHis tag, His tag, Hexa-His tag, Polyhistidine tag, His6 tag, 6His tag |
| Function The 6×His tag enables purification and detection of recombinant proteins through coordination of histidine residues with immobilized metal ions such as nickel, cobalt, copper, or zinc. |
| Description The 6×His tag is a synthetic peptide tag consisting of six consecutive histidine residues. It is one of the most widely used affinity tags for recombinant protein purification and detection. |
| Related entities relationship: binds_to; entity: Nickel ion relationship: binds_to; entity: Ni-NTA relationship: binds_to; entity: Cobalt affinity resin relationship: modified_form_of; entity: 8×His tag relationship: modified_form_of; entity: 10×His tag |
| Keywords Affinity tag, Protein purification, IMAC, Recombinant protein, Protein detection |
| Sequence HHHHHH |
| Structure The 6×His tag consists of six consecutive histidine residues (HHHHHH) and is typically fused to the N- or C-terminus of recombinant proteins. |
| Function The histidine residues coordinate transition metal ions, enabling affinity purification and detection of tagged proteins. |
| Purification His-tagged proteins are commonly purified using immobilized metal affinity chromatography (IMAC), including Ni-NTA and cobalt-based resins. |
| Detection His-tagged proteins can be detected using anti-His antibodies, anti-polyhistidine antibodies, nickel conjugates, or IMAC reagents. |
| Research relevance The 6×His tag is one of the most widely used recombinant protein tags because of its small size, minimal impact on protein folding, and compatibility with rapid purification workflows. |
Data
 |
| Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-6-His antibody 7632 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
|
| Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-6-His antibody 7632 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
 |
| Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, rabbit anti-6-His antibody 7632 used for WB at 0.04 µg /ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg /ml) and chemiluminescence with an exposure time of 10 seconds. |
|
| Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, biotin-conjugated, rabbit anti-6-His antibody 7632 used for WB at 0.04 µg /ml (1:25,000). Detection: Streptavidin-HRP (0.1 µg /ml) and chemiluminescence with an exposure time of 10 seconds. |
FAQ & Publications
Frequently Asked Questions
What applications is the rabbit anti-6 His polyclonal antibody 7632 validated for?
This antibody is suitable and tested for Western Blotting (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), and ELISA applications.
How should the rabbit anti-6 His polyclonal antibody 7632 be stored to maintain stability?
The antibody is stable for at least one year when stored at 2-8°C in its provided storage buffer, which is PBS at pH 7.2 with 0.09% sodium azide.
What is the host species and clonality of the anti-6 His antibody 7632?
This antibody is a rabbit polyclonal IgG antibody.
What is the recommended dilution for using this antibody in Western blotting?
For Western blotting, it is recommended to use the antibody at a dilution range of 1:1,000 to 1:20,000.
What immunogen was used to generate the rabbit anti-6 His polyclonal antibody 7632?
The immunogen used was the 6-His peptide sequence (HHHHHH) conjugated to keyhole limpet hemocyanin (KLH).
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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