ICC/IF image mouse anti Actin (pan) monoclonal antibody 5J11 7047

mouse anti-Actin (pan) monoclonal antibody (5J11) 7047

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to Actin (pan)
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat, cow, pig
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

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SKU: 7047parent Categories: antibody, loading control Tag: Actin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	cow, human, mouse, pig, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-Actin (pan) monoclonal antibody (5J11) 7047

antibody
Database link: human actins P62736,P63267,P60709,P63261,P68133,P68032 mouse actins P62737 P63268,P68033,P60710,P68134, rat actins P62738,P63268,P63269,P68035,P60711,P68136
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:1000 IF/ICC or IHC: 1:500-1000
Immunogen Actin preparation from bovine brain
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance

Actin, a highly conserved protein, is widely recognized as an essential loading control in scientific research, particularly in the field of cell biology and molecular biology. As a structural component of the cytoskeleton, actin is present in virtually all eukaryotic cells. Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Many of these processes are mediated by extensive and intimate interactions of actin with cellular membranes. Its ubiquitous expression and relatively stable levels make it an ideal reference protein for normalizing protein loading in Western blotting and other protein analysis techniques. Actin' abundance and consistent expression ensure that its quantity remains relatively constant across different experimental conditions and sample variations.

Actin isoforms are distinct variants of the actin protein found in eukaryotic cells, and they play crucial roles in various cellular processes. One of the primary differences between actin isoforms lies in their tissue-specific distribution. For instance, skeletal muscle cells predominantly express the α-actin isoform, while smooth muscle cells mainly contain the γ-actin isoform. These tissue-specific distributions are essential for the specialized functions of muscle cells. Moreover, actin isoforms can also differ in their post-translational modifications. For example, γ-actin is more prone to undergo acetylation than α-actin. Additionally, actin isoforms can exhibit variations in their kinetic properties, such as rates of polymerization and depolymerization, which can influence their roles in processes like cell motility or cytoskeletal stability. Furthermore, some actin isoforms may interact with different binding partners or regulatory proteins, further diversifying their functions within the cell. These differences in tissue distribution, post-translational modifications, kinetic properties, and protein interactions contribute to the versatility and specificity of actin isoforms in various cellular processes and underline their importance in maintaining cellular structure and function.

This antibody recognizes multiple actin isoforms and can be used as a loading control when run alongside proteins of interest with different and resolvable molecular weights and ideally in combination with antibodies of same host and when using a secondary antibody.

Click for more on: loading controls and Actin

Protein names
PANACT

Data

Immunofluorescent analysis of HeLa cells stained with mAb against actin, 7047, in green, costained with EnCor chicken pAb against vimentin, 6886, in red. The actin antibody labels the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. The vimentin antibody stains a different cytoskeletal network, the intermediate or 10nm filaments. The blue is DAPI staining of nuclear DNA.

IHC-image-mouse-anti-Actin (pan)-monoclonal-antibody-5J11-7047

Chromogenic immunostaining of a formalin fixed paraffin embedded human brain cortex section with mouse mAb to actin, 7047, dilution 1:1,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 7047 antibody labels the cytoplasm of all cell types tested. This antibody performs well in staining both 4% PFA and standard NBF fixed tissues. Mouse select image

GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with 7047. The 7047 antibody binds all six proteins strongly, so we conclude that it is a pan specific actin antibody. This is not a surprising finding given the high degree of identity between the six proteins, see below. These constructs can be purchased from Novus Biologicals. The HGNC names for the actin isotypes are two α gene products. α-1 actin and α-2, which are enteric and smooth muscle actins (ACTG1 and ACTG2). The single beta-actin is ACTB, and alpha-skeletal actin, alpha-vascular smooth muscle actin and alpha-cardiac muscle are ACTA1, ACTA2, and ACTC1. The amino acid sequences of the six human actin gene products are 94-97% identical, so antibodies which bind all six gene products are to be expected. A sequence alignment can be downloaded from here.

Western blot analysis of tissue and cell lysates probed with mouse mAb against actin, 7047, in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with EnCor chicken pAb to UCHL-1 , a marker of neuronal lineage cells, in green.

Publications

pmid	title	authors	citation
37310811	Oncostatin-M and OSM-Receptor Feed-Forward Activation of MAPK Induces Separable Stem-like and Mesenchymal Programs.	Kelsey L Polak, Ilaria Tamagno, Neetha Parameswaran, Jacob Smigiel, E Ricky Chan, Xueer Yuan, Brenda Rios, Mark W Jackson	Mol Cancer Res 21:975-990
33839158	The C99 domain of the amyloid precursor protein resides in the disordered membrane phase.	Ricardo Capone, Ajit Tiwari, Arina Hadziselimovic, Yelena Peskova, James M Hutchison, Charles R Sanders, Anne K Kenworthy	J Biol Chem 296:100652
33175436	Neuron-specific cilia loss differentially alters locomotor responses to amphetamine in mice.	Carlos Ramos, Jont� B Roberts, Kalene R Jasso, Tyler W Ten Eyck, Thomas Everett, Patricia Pozo, Barry Setlow, Jeremy C McIntyre	J Neurosci Res 99:827-842
29321224	Reconsidering an active role for G-actin in cytoskeletal regulation.	Kristen Skruber, Tracy-Ann Read, Eric A Vitriol	J Cell Sci 131:N/A
27133808	Unravelling the Actin Cytoskeleton: A New Competitive Edge?	Andrew J Davidson, Will Wood	Trends Cell Biol 26:569-576

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.