rabbit anti-ERK1 monoclonal antibody 9031

$409.00

Antibody summary

Rabbit monoclonal to ERK1
Suitable for: WB
Reacts with: human, mouse
Isotype: IgG1
100 µg

SKU: 9031 Categories: antibody, loading control Tag: ERK1

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	monoclonal
concentration	1 mg/mL
applications	WB
available sizes	100 µg

rabbit anti-ERK1 monoclonal antibody 9031

antibody
Database link: human ERK1 P27361
Tested applications WB
Recommended dilutions WB: 1:1000-10000
Immunogen Recombinant full length ERK1 protein.
Size and concentration 100µg and 1 mg/mL
Form liquid
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Rabbit polycolonal - Isotype Control

target relevance
ERK1 (Extracellular signal-Regulated Kinase 1) is a highly conserved member of the mitogen-activated protein kinase (MAPK) family, playing a crucial role in cellular signaling pathways. It is a serine/threonine kinases that becomes activated in response to a variety of extracellular stimuli, including growth factors, hormones, and stress. Upon activation, ERK1 translocates to the nucleus, where it phosphorylates numerous downstream targets, including transcription factors and other kinases. Through these phosphorylation events, ERK1 regulates a wide range of cellular processes, such as cell proliferation, differentiation, survival, and apoptosis. Dysregulation of ERK1 signaling has been implicated in various diseases, including cancer. neurodegenerative disorders, and inflammatory conditions. This antibody can be used as a loading control when evaluating post-translational modification of ERK1 and other cell singalling kinases and their modifications. Click for more on: loading controls and ERK1
Protein names Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1)
Gene names MAPK3,MAPK3 ERK1 PRKM3
Protein family Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamil
Mass 43136Da
Catalytic activity CATALYTIC ACTIVITY: Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.24; Evidence={ECO:0000269\|PubMed:35216969}; CATALYTIC ACTIVITY: Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.24;
Structure SUBUNIT: Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14 (By similarity). Interacts with ADAM15, ARRB2, CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, and SGK1. Interacts with PEA15 and MKNK2 (By similarity). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with TPR. Interacts with CDKN2AIP. Interacts with HSF1 (via D domain and preferentially with hyperphosphorylated form); this interaction occurs upon heat shock (PubMed:10747973). Interacts with CAVIN4 (By similarity). Interacts with GIT1; this interaction is necessary for MAPK3 localization to focal adhesions (By similarity). Interacts with ZNF263 (PubMed:32051553). Interacts with EBF4. {ECO:0000250\|UniProtKB:P21708, ECO:0000250\|UniProtKB:Q63844, ECO:0000269\|PubMed:10393181, ECO:0000269\|PubMed:10521408, ECO:0000269\|PubMed:10747973, ECO:0000269\|PubMed:11912194, ECO:0000269\|PubMed:12356731, ECO:0000269\|PubMed:15616583, ECO:0000269\|PubMed:16581800, ECO:0000269\|PubMed:18296648, ECO:0000269\|PubMed:18435604, ECO:0000269\|PubMed:18794356, ECO:0000269\|PubMed:19060905, ECO:0000269\|PubMed:19447520, ECO:0000269\|PubMed:24825908, ECO:0000269\|PubMed:32051553, ECO:0000269\|PubMed:35939714}.; SUBUNIT: (Microbial infection) Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. {ECO:0000269\|PubMed:8794306}.
Post-translational modification PTM: Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204. {ECO:0000250, ECO:0000269\|PubMed:17274988, ECO:0000269\|PubMed:18983981, ECO:0000269\|PubMed:19060905, ECO:0000269\|PubMed:19265199, ECO:0000269\|PubMed:19494114}.; PTM: Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination; leading to activation. Deubiquitinated by CYLD. {ECO:0000269\|PubMed:34497368}.
Target Relevance information above includes information from UniProt accession: P27361
The UniProt Consortium

Data

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Detection of human ERK1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ERK1 antibody used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 30 seconds.

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Detection of human ERK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ERK1 antibody used for IP at 3 µg/mg lysate. For blotting immunoprecipitated ERK1, the antibody was used at 1.0 µg/ml. Detection: Chemiluminescence with exposure time of 3 seconds.

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Detection of human ERK1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERK1 antibody used at a dilution of 1:200 (1µg/ml). Detection: DAB

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.