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West Nile Virus IgM ELISA kit-SERION classic ESR141M

$364.00

Summary

Virion/Serion Diagnostic Kit for research use (RUO)
West Nile Virus IgM ELISA Kit
Suitable for IgM detection
Ready-to-use
96 tests

SKU: ESR141M Category: Virion/Serion kits Tags: ELISA, IgM, West Nile Virus

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	West Nile Virus reactive IgM
species reactivity	West Nile Virus
applications	ELISA
assay type	Indirect & quantitative
available sizes	96 tests

West Nile Virus IgM ELISA Kit ESR141M

Assay type
Indirect ELISA

Research area
Infectious Disease

Sample type
Serum, plasma, whole blood

Notes
Pretreatment of samples with RF-Absorbent (Z200) is recommended for use with IgM ELISA kits to eliminate presence of sample rheumatoid factors and possible false negative results.

Components

Break apart microtiter test strips each with antigen coated single wells	8 x 12 (96 Total)
Standard serum (ready-to-use)	2 x 2 mL
Negative control serum (ready-to-use)	2 mL
Anti-human-IgM-conjugate (ready-to-use)	13 mL
Washing solution concentrate (sufficient for 1000ml)	33.3 mL
Dilution buffer	2 x 50 mL
Stopping solution	15 mL
Substrate (ready-to-use)	13 mL
Quality control certificate with standard curve and evaluation table	1

Storage
Store at 2-8°C.

Associated products
West Nile Virus Human IgG Assay Control (BC141G)
West Nile Virus Human IgM Assay Control (BC141M)
West Nile Virus IgG ELISA Kit (ESR141G)
West Nile Virus IgM ELISA Kit (ESR141M)

target relevance

human antibody anti-West Nile Virus

West Nile Virus

Description
Virus that causes West Nile fever and neuroinvasive disease

Structure
West Nile virus (WNV) is an enveloped, positive-sense, single-stranded RNA virus belonging to the genus Flavivirus within the family Flaviviridae. The virus is closely related to other medically important flaviviruses, including Japanese encephalitis virus, St. Louis encephalitis virus, Dengue virus, and Zika virus. West Nile virus is maintained in nature through transmission cycles involving mosquitoes, primarily Culex species, and avian reservoir hosts. Humans and horses are considered incidental or dead-end hosts because they typically do not develop sufficient viremia to sustain transmission. Following mosquito-borne inoculation, the virus can disseminate to multiple tissues and, in some cases, invade the central nervous system. Since its introduction into North America in 1999, West Nile virus has become one of the most important mosquito-borne viral pathogens in the Western Hemisphere.

Diagnosis
The diagnosis of West Nile virus infection is performed through serological and molecular methods. Detection of West Nile virus-specific IgM antibodies in serum or cerebrospinal fluid using ELISA is the most commonly employed diagnostic approach and is particularly useful in patients with neurological disease. Confirmation may be obtained through plaque reduction neutralization testing (PRNT) to distinguish West Nile virus infection from other flaviviral infections. Reverse transcription polymerase chain reaction (RT-PCR) can detect viral RNA during the early phase of infection but is generally less sensitive because viremia is often transient and low-level. Cerebrospinal fluid analysis in neuroinvasive disease typically demonstrates lymphocytic pleocytosis and elevated protein concentrations. Laboratory confirmation is important because clinical symptoms overlap with numerous other viral infections and causes of encephalitis.

Symptoms
Approximately 80% of West Nile virus infections are asymptomatic. Symptomatic individuals typically develop illness following an incubation period of approximately two to fourteen days. West Nile fever commonly presents with fever, headache, fatigue, malaise, muscle aches, joint pain, nausea, vomiting, and occasionally a maculopapular rash. Most patients recover completely within days to weeks. A small proportion of infected individuals, particularly elderly or immunocompromised patients, develop neuroinvasive disease characterized by meningitis, encephalitis, or acute flaccid paralysis. Neurological manifestations may include severe headache, neck stiffness, confusion, altered mental status, tremors, seizures, muscle weakness, and paralysis. Severe neuroinvasive disease can result in long-term neurological deficits, respiratory failure, or death.

Data

FAQ & Publications

Frequently Asked Questions

What type of samples can be used with the West Nile Virus IgM ELISA Kit ESR141M?

The kit is compatible with serum, plasma, and whole blood samples for the detection of West Nile Virus reactive IgM antibodies.

Is there any recommended pretreatment to improve the accuracy of IgM detection using this kit?

Yes, pretreatment of samples with RF-Absorbent (Z200) is recommended to eliminate rheumatoid factors that may cause false negative results in IgM ELISA assays.

How should the West Nile Virus IgM ELISA Kit ESR141M be stored to maintain its stability?

The kit components should be stored refrigerated at 2-8°C to ensure proper stability and performance.

What is the assay format and detection principle of the West Nile Virus IgM ELISA Kit ESR141M?

The kit utilizes an indirect ELISA assay format that quantitatively detects IgM antibodies reactive to West Nile Virus antigens.

How many tests are provided in one kit of the West Nile Virus IgM ELISA Kit ESR141M?

Each kit contains 96 tests, provided as 8 strips of 12 antigen-coated microtiter wells.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
ESR141M protocol

Documents

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West Nile Virus IgM ELISA kit-SERION classic ESR141M

RF-Absorbent Z200 for use with IgM ELISA kits

Data

FAQ & Publications

Protocols

Documents

Related Technical Resources & Posts

Reviews

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