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Brucella IgM ELISA kit-SERION classic ESR116M

$364.00

Summary

Virion/Serion Diagnostic Kit for research use (RUO)
Brucella IgM ELISA Kit
Suitable for IgM detection
Ready-to-use
96 tests

SKU: ESR116M Category: Virion/Serion kits Tags: Brucella, ELISA, IgM

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Brucella reactive IgM
species reactivity	Brucella
applications	ELISA
assay type	Indirect & quantitative
available sizes	96 tests

Brucella IgM ELISA Kit ESR116M

Assay type
Indirect ELISA

Research area
Infectious Disease

Sample type
Serum, plasma, whole blood

Notes
Pretreatment of samples with RF-Absorbent (Z200) is recommended for use with IgM ELISA kits to eliminate presence of sample rheumatoid factors and possible false negative results.

Components

Break apart microtiter test strips each with antigen coated single wells	8 x 12 (96 Total)
Standard serum (ready-to-use)	2 x 2 mL
Negative control serum (ready-to-use)	2 mL
Anti-human-IgM-conjugate (ready-to-use)	13 mL
Washing solution concentrate (sufficient for 1000ml)	33.3 mL
Dilution buffer	2 x 50 mL
Stopping solution	15 mL
Substrate (ready-to-use)	13 mL
Quality control certificate with standard curve and evaluation table	1

Storage
Store at 2-8°C.

Associated products
Brucella abortus Antigen (BA116VS)
Brucella IgA Control Serum (BC116A)
Brucella IgG Control Serum (BC116G)
Brucella IgM Control Serum (BC116M)
Brucella IgA ELISA Kit (ESR116A)
Brucella IgG ELISA Kit (ESR116G)
Brucella IgM ELISA Kit (ESR116M)

target relevance

human antibody anti-Brucella

Brucella

Description
Bacterial genus that causes brucellosis (undulant fever)

Structure
Brucella is a genus of small, Gram-negative, facultative intracellular coccobacilli belonging to the family Brucellaceae. Members of the genus are zoonotic pathogens that infect a wide range of domestic and wild animals, including cattle, sheep, goats, pigs, dogs, and wildlife species. The most important human pathogens include Brucella melitensis, Brucella abortus, Brucella suis, and Brucella canis. Brucella species are highly adapted to survival and replication within macrophages and other phagocytic cells, enabling persistent infection and evasion of host immune responses. Transmission to humans commonly occurs through direct contact with infected animals, inhalation of contaminated aerosols, or consumption of unpasteurized dairy products. Due to their low infectious dose and ability to become aerosolized, Brucella species are considered important laboratory-acquired pathogens.

Diagnosis
The diagnosis of brucellosis is performed through microbiological, serological, and molecular methods. Isolation of Brucella organisms from blood, bone marrow, or tissue specimens remains the definitive diagnostic method, although culture may require prolonged incubation and specialized laboratory precautions. Serological testing is widely used and includes standard agglutination tests, ELISA, complement fixation tests, and other assays for the detection of Brucella-specific antibodies. Polymerase chain reaction (PCR) provides rapid and sensitive detection of Brucella DNA and may be particularly useful in chronic or complicated cases. Laboratory findings often include anemia, leukopenia, thrombocytopenia, and elevated inflammatory markers. Because symptoms are frequently nonspecific, laboratory confirmation is essential for diagnosis.

Symptoms
Brucellosis typically develops following an incubation period of one to four weeks but may occur months after exposure. The disease is characterized by fever, chills, profuse night sweats, fatigue, malaise, headache, muscle aches, joint pain, and loss of appetite. Fever may follow a recurrent or undulating pattern, giving rise to the term 'undulant fever.' Chronic infection can involve multiple organ systems and may result in arthritis, spondylitis, osteomyelitis, epididymo-orchitis, hepatitis, endocarditis, and neurological complications. Endocarditis is the most serious complication and is the leading cause of brucellosis-associated mortality. Without appropriate treatment, symptoms may persist for months or years and can cause significant long-term disability.

Data

FAQ & Publications

Frequently Asked Questions

What sample types are compatible with the Brucella IgM ELISA Kit ESR116M for detecting Brucella-specific IgM antibodies?

The Brucella IgM ELISA Kit ESR116M is compatible with serum, plasma, and whole blood samples for the detection of Brucella-reactive IgM antibodies.

How should samples be pretreated to improve the specificity of IgM detection using this ELISA kit?

It is recommended to pretreat samples with RF-Absorbent (Z200) to remove IgM rheumatoid factors from serum and plasma, which helps eliminate potential false negative results and improves signal specificity in the assay.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
ESR116M protocol

Documents

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Brucella IgM ELISA kit-SERION classic ESR116M

RF-Absorbent Z200 for use with IgM ELISA kits

Data

FAQ & Publications

Protocols

Documents

Related Technical Resources & Posts

Reviews

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