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Coxiella burnetii IgM Phase II ELISA kit-SERION classic ESR1312M

$355.00

Summary

Virion/Serion Diagnostic Kit for research use (RUO)
Coxiella burnetii IgM Phase II ELISA Kit
Suitable for IgM detection
Ready-to-use
96 tests

SKU: ESR1312M Category: Virion/Serion kits Tags: Coxiella burnetii, ELISA, IgM

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Coxiella burnetii reactive IgM Phase II
species reactivity	Coxiella burnetii (Q fever)
applications	ELISA
assay type	Indirect & quantitative
available sizes	96 tests

Coxiella burnetii IgM Phase II ELISA Kit ESR1312M

Assay type
Indirect ELISA

Research area
Infectious Disease

Sample type
Serum, plasma, whole blood

Notes
Pretreatment of samples with RF-Absorbent (Z200) is recommended for use with IgM ELISA kits to eliminate presence of sample rheumatoid factors and possible false negative results.

Components

Break apart microtiter test strips each with antigen coated single wells	8 x 12 (96 Total)
Standard serum (ready-to-use)	2 x 2 mL
Negative control serum (ready-to-use)	2 mL
Anti-human-IgM-conjugate (ready-to-use)	13 mL
Washing solution concentrate (sufficient for 1000ml)	33.3 mL
Dilution buffer	2 x 50 mL
Stopping solution	15 mL
Substrate (ready-to-use)	13 mL
Quality control certificate with standard curve and evaluation table	1

Storage
Store at 2-8°C.

Associated products
Coxiella burnetii Phase 2 IgG Control Serum (BC1312G)
Coxiella burnetii Phase 2 IgM Control Serum (BC1312M)
Coxiella burnetii IgA Phase I ELISA Kit (ESR1311A)
Coxiella burnetii IgG Phase I ELISA Kit (ESR1311G)
Coxiella burnetii IgG Phase II ELISA Kit (ESR1312G)
Coxiella burnetii IgM Phase II ELISA Kit (ESR1312M)

target relevance

human antibody anti-Coxiella burnetii1

Coxiella burnetii

Description
Bacterium that causes Q fever

Structure
Coxiella burnetii is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. The genus Coxiella is morphologically similar to Rickettsia, but with a variety of genetic and physiological differences. C. burnetii is a small Gram-negative, coccobacillary bacterium that is highly resistant to environmental stresses such as high temperature, osmotic pressure, and ultraviolet light. These characteristics are attributed to a small cell variant form of the organism that is part of a biphasic developmental cycle, including a more metabolically and replicatively active large cell variant form. It can survive standard disinfectants, and is resistant to many other environmental changes like those presented in the phagolysosome.

Diagnosis
The diagnosis of Q fever is performed by the demonstration of specific antibodies directed against Coxiella burnetii. Due to variations in the lipopolysaccharide (LPS) structure on the surface of the pathogen, as the disease enters the chronic state, a serological differentiation of acute from chronic infections is possible. Due to the high sensitivity and specificity, the use of ELISA immunoassays is recommended by the World Health Organization (WHO). Following the regular course of an acute primary infection, specific IgM and IgG antibodies directed against the immunogenic phase 2 antigens can be demonstrated. IgG antibodies directed against phase 2 antigens often persist over several years. In the lead-up to a chronic infection, IgG and IgA antibodies directed against the phase 1 antigens appear, which are of diagnostic value particularly for the diagnosis of Q fever endocarditis

Symptoms
Coxiella burnetii is a gram-negative, aerobic coccobacillus of the Coxiellaceae family. The causative agent of the so called Q fever is extremely infectious and very resistant to environmental factors. <br><br>Approximately half of infected individuals exhibit no clinical symptoms. The most common manifestation following an incubation period of two to three weeks, are mild flulike symptoms with abrupt onset of fever, malaise, severe headache, myalgia, loss of appetite, dry cough, chest pain and chill, more seldom accompanied by gastrointestinal symptoms such as nausea, vomiting and diarrhea. During its course, the disease can progress to an atypical pneumonia, which may result in a life-threatening acute respiratory distress syndrome (ARDS). More seldom, Q fever presents as granulomatous hepatitis with inflammation of the liver. In rare cases, the disease takes a chronic course and presents as an inflammation of the inner lining of the heart muscle (endocarditis) or of the heart sac (pericarditis), which is usually fatal if untreated.

Data

FAQ & Publications

Frequently Asked Questions

What is the primary application of the Coxiella burnetii IgM Phase II ELISA Kit ESR1312M?

The Coxiella burnetii IgM Phase II ELISA Kit ESR1312M is designed for indirect and quantitative detection of IgM antibodies specific to Coxiella burnetii Phase II antigens, primarily for research use in infectious disease studies.

Which sample types are compatible with this ELISA kit?

This ELISA kit is compatible with serum, plasma, and whole blood samples for the detection of Coxiella burnetii IgM Phase II antibodies.

What storage conditions are recommended for maintaining the Coxiella burnetii IgM Phase II ELISA Kit?

The kit components should be stored at 2-8°C to ensure stability and optimal performance.

Is there a recommended pretreatment step for samples used with this IgM ELISA kit?

Yes, pretreatment of samples with RF-Absorbent (Z200) is recommended to remove rheumatoid factors from serum and plasma samples, which helps to prevent false negative results and improves signal specificity.

What components are included in the Coxiella burnetii IgM Phase II ELISA Kit ESR1312M?

The kit includes 8 strips of 12 antigen-coated wells (96 total), ready-to-use standard serum, negative control serum, anti-human-IgM conjugate, washing solution concentrate, dilution buffer, stopping solution, substrate, and a quality control certificate with standard curve and evaluation table.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
ESR1312M protocol

Documents

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Coxiella burnetii IgM Phase II ELISA kit-SERION classic ESR1312M

RF-Absorbent Z200 for use with IgM ELISA kits

Data

FAQ & Publications

Protocols

Documents

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Reviews

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