Immunohistochemistry (IHC) : Human prostate stained with anti-PSAP antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of benign prostate glands.

rabbit anti-PSAP monoclonal antibody (ZR443) 6350

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to PSAP
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Human prostate
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6350parent Category: antibody Tags: anatomy, monoclonal, PSAP, Urinary Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-PSAP monoclonal antibody ZR443 6350

antibody
Database link: human O14601
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Human prostate
Immunogen Recombinant human ACPP fragment
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Prostatic acid phosphatase (PAP) (EC 3.1.3.2) (5'-nucleotidase) (5'-NT) (EC 3.1.3.5) (Acid phosphatase 3) (Ecto-5'-nucleotidase) (Protein tyrosine phosphatase ACP3) (EC 3.1.3.48) (Thiamine monophosphatase) (TMPase) [Cleaved into: PAPf39]
Gene names ACP3,ACP3 ACPP
Protein family Histidine acid phosphatase family
Mass 44566Da
Function FUNCTION: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed:10506173, PubMed:15280042, PubMed:20498373, PubMed:9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed:10506173, PubMed:15280042). {ECO:0000269\|PubMed:10506173, ECO:0000269\|PubMed:15280042, ECO:0000269\|PubMed:20498373, ECO:0000269\|PubMed:9584846}.; FUNCTION: [Isoform 2]: Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling (PubMed:20498373). In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity). {ECO:0000250\|UniProtKB:Q8CE08, ECO:0000269\|PubMed:20498373}.; FUNCTION: [PAPf39]: (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed:18083097, PubMed:19451623, PubMed:19897482). SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed:19451623). Target cell attachment and enhancement of HIV infection is inhibited by surfen (PubMed:19897482). Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed:19403677). {ECO:0000269\|PubMed:18083097, ECO:0000269\|PubMed:19403677, ECO:0000269\|PubMed:19451623, ECO:0000269\|PubMed:19897482}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=a phosphate monoester + H2O = an alcohol + phosphate; Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879, ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2; Evidence={ECO:0000269\|PubMed:10506173, ECO:0000269\|PubMed:15280042, ECO:0000269\|PubMed:9584846}; CATALYTIC ACTIVITY: Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 1-(9Z-octadecenoyl)-sn-glycerol + phosphate; Xref=Rhea:RHEA:39835, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:74544, ChEBI:CHEBI:75757; Evidence={ECO:0000269\|PubMed:10506173}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39836; Evidence={ECO:0000305\|PubMed:10506173}; CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5; Evidence={ECO:0000250\|UniProtKB:Q8CE08}; CATALYTIC ACTIVITY: Reaction=O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:20101, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:61978; EC=3.1.3.48; Evidence={ECO:0000269\|PubMed:20498373};
Subellular location SUBCELLULAR LOCATION: [Isoform 1]: Secreted {ECO:0000305\|PubMed:17638863}.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane {ECO:0000269\|PubMed:17638863, ECO:0000269\|PubMed:17897319, ECO:0000269\|PubMed:20498373}; Single-pass type I membrane protein {ECO:0000255}. Lysosome membrane {ECO:0000269\|PubMed:17638863, ECO:0000269\|PubMed:17897319}; Single-pass type I membrane protein {ECO:0000255}. Nucleus {ECO:0000269\|PubMed:20498373}. Cytoplasm, cytosol {ECO:0000269\|PubMed:20498373}. Note=Appears to shuttle between the cell membrane and intracellular vesicles. Colocalizes with FLOT1 at cell membrane and in intracellular vesicles (PubMed:17638863). Colocalizes with LAMP2 on the lysosome membrane (PubMed:17897319). {ECO:0000269\|PubMed:17638863, ECO:0000269\|PubMed:17897319}.
Tissues TISSUE SPECIFICITY: Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues. {ECO:0000269\|PubMed:17638863, ECO:0000269\|PubMed:21487525}.; TISSUE SPECIFICITY: [Isoform 2]: Widely expressed. Expressed in the sarcolemma of skeletal muscle. {ECO:0000269\|PubMed:17638863}.
Structure SUBUNIT: Homodimer; dimer formation is required for phosphatase activity. {ECO:0000250\|UniProtKB:P20646}.
Post-translational modification PTM: N-glycosylated. High mannose content, partially sialylated and fucosylated biantennary complex. Also fucosylated with partially sialylated triantennary complex oligosaccharides. {ECO:0000269\|PubMed:10639192, ECO:0000269\|PubMed:12525165}.; PTM: Proteolytically cleaved in seminal fluid to produce several peptides. Peptide PAPf39, the most prominent, forms amyloid beta-sheet fibrils, SEVI (semen-derived enhancer of viral infection). {ECO:0000269\|PubMed:18083097}.
Target Relevance information above includes information from UniProt accession: P15309
The UniProt Consortium

Data

Human prostate stained with anti-PSAP antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of benign prostate glands.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.