Immunohistochemistry (IHC) : Human tonsil stained with anti-PAX-5 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining in B cells in follicle.

rabbit anti-PAX-5 monoclonal antibody (ZR268) 6432

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to PAX-5
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Tonsil
Visualization: Nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6432parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, Hematopathology, monoclonal, PAX-5

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-PAX-5 monoclonal antibody ZR268 6432

antibody
Database link: human Q02548
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Tonsil
Immunogen Recombinant human full-length PAX-5 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Homo sapiens PAX5 Paired box protein Pax-5
Protein names Paired box protein Pax-5
Alternative names B-cell-specific transcription factor
Gene names PAX5
Function Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed:10811620, PubMed:27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed:10811620, PubMed:27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed:32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed:32612238)
Subcellular location Nucleus
Structure (Microbial infection) Interacts (via N-terminus) with Epstein-Barr virus protein BZLF1 (via C-terminus); this interaction inhibits BZLF1-mediated lytic viral reactivation (PubMed:23678172). Interacts also with EBNA1; this interaction promotes EBNA1-dependent transcription (PubMed:31941781)
Post-translational modification O-glycosylated Phosphorylated by SYK. This phosphorylation plays an important role in the abolition of BLIMP1 repression by PAX5 in order to trigger plasma cell differentiation
Involvement in disease Leukemia, acute lymphoblastic, 3 A subtype of acute leukemia, a cancer of the white blood cells. Acute lymphoblastic anemia is a malignant disease of bone marrow and the most common malignancy diagnosed in children. The malignant cells are lymphoid precursor cells (lymphoblasts) that are arrested in an early stage of development. The lymphoblasts replace the normal marrow elements, resulting in a marked decrease in the production of normal blood cells. Consequently, anemia, thrombocytopenia, and neutropenia occur to varying degrees. The lymphoblasts also proliferate in organs other than the marrow, particularly the liver, spleen, and lymphnodes.
Keywords 3D-structure, Alternative splicing, Chromosomal rearrangement, Developmental protein, Differentiation, Disease variant, DNA-binding, Glycoprotein, Neurogenesis, Nucleus, Paired box, Proteomics identification, Proto-oncogene, Reference proteome, Spermatogenesis, Transcription, Transcription regulation
Sequence MDLEKNYPTPRTSRTGHGGVNQLGGVFVNGRPLPDVVRQRIVELAHQGVRPCDISRQLRV SHGCVSKILGRYYETGSIKPGVIGGSKPKVATPKVVEKIAEYKRQNPTMFAWEIRDRLLA ERVCDNDTVPSVSSINRIIRTKVQQPPNQPVPASSHSIVSTGSVTQVSSVSTDSAGSSYS ISGILGITSPSADTNKRKRDEGIQESPVPNGHSLPGRDFLRKQMRGDLFTQQQLEVLDRV FERQHYSDIFTTTEPIKPEQTTEYSAMASLAGGLDDMKANLASPTPADIGSSVPGPQSYP IVTGRDLASTTLPGYPPHVPPAGQGSYSAPTLTGMVPGSEFSGSPYSHPQYSSYNDSWRF PNPGLLGSPYYYSAAARGAAPPAAATAYDRH
UniProt accession: Q02548

Data

Human tonsil stained with anti-PAX-5 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining in B cells in follicle.

FAQ & Publications

Frequently Asked Questions

What species does the rabbit anti-PAX-5 monoclonal antibody (ZR268) specifically react with?

This antibody specifically reacts with human tissues.

How should the rabbit anti-PAX-5 monoclonal antibody be stored to maintain its stability?

For short-term storage, keep the antibody at 2-8°C. For long-term storage, it should be kept at -20°C while avoiding freeze/thaw cycles to preserve its quality.

Is the rabbit anti-PAX-5 monoclonal antibody suitable for immunohistochemistry on formalin-fixed, paraffin-embedded tissues?

Yes, this antibody is validated for immunohistochemistry applications using formalin-fixed, paraffin-embedded tissue samples.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.