Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human spleen stained with anti-perforin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic granular staining of cytotoxic T cells

rabbit anti-Perforin monoclonal antibody (ZR270) 6330

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to Perforin
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Spleen
Visualization: Granular cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6330parent Category: antibody Tags: anatomy, Hematopathology, monoclonal, Perforin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-Perforin monoclonal antibody ZR270 6330

antibody
Database link: human P14222
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Spleen
Immunogen Recombinant human Perforin protein fragment (around aa 413-552)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Perforin-1 (P1) (Cytolysin) (Lymphocyte pore-forming protein) (PFP)
Gene names PRF1,PRF1 PFP
Protein family Complement C6/C7/C8/C9 family
Mass 61377Da
Function FUNCTION: Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:20889983, PubMed:21037563, PubMed:24558045, PubMed:9058810, PubMed:9164947). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:20889983, PubMed:21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:24558045, PubMed:32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed:24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity). {ECO:0000250\|UniProtKB:P10820, ECO:0000269\|PubMed:20038786, ECO:0000269\|PubMed:20225066, ECO:0000269\|PubMed:20889983, ECO:0000269\|PubMed:21037563, ECO:0000269\|PubMed:24558045, ECO:0000269\|PubMed:32299851, ECO:0000269\|PubMed:9058810, ECO:0000269\|PubMed:9164947}.
Subellular location SUBCELLULAR LOCATION: Cytolytic granule {ECO:0000269\|PubMed:20038786, ECO:0000269\|PubMed:24088571}. Secreted. Cell membrane {ECO:0000269\|PubMed:20889983, ECO:0000269\|PubMed:21037563}; Multi-pass membrane protein {ECO:0000269\|PubMed:20889983, ECO:0000269\|PubMed:21037563}. Endosome lumen {ECO:0000269\|PubMed:20038786}. Note=Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell (PubMed:20038786). Inserts into the cell membrane of target cells and forms pores (PubMed:20889983). Membrane insertion and pore formation requires a major conformation change (PubMed:20889983). May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes (PubMed:20038786). {ECO:0000269\|PubMed:20038786, ECO:0000269\|PubMed:20889983}.
Structure SUBUNIT: Monomer, as soluble protein (PubMed:20889983, PubMed:21037563). Homooligomer; homooligomerizes to form a pore-forming ring (PubMed:20889983, PubMed:21037563). {ECO:0000269\|PubMed:20889983, ECO:0000269\|PubMed:21037563}.
Post-translational modification PTM: N-glycosylated. {ECO:0000250\|UniProtKB:P10820}.
Domain DOMAIN: Perforin consists of three domains: (1) the MACPF domain, which includes the central machinery of pore formation, (2) the EGF-like domain, which forms a 'shelf-like' assembly connecting the MACPF and C2 domains, and (3) the C2 domain, which mediates calcium-dependent binding to lipid membranes. The C2 domain is critical for initial calcium-dependent interaction with lipid membranes of the target cell: calcium-binding causes a significant structural rearrangement, leading to oligomerization and deployment of the two transmembrane beta-strands (named CH1/TMH1 and CH2/TMH2) that enter the membrane as amphipathic beta-hairpins. The third calcium-binding site (Ca(2+) 3), which constitutes the weakest affinity site, triggers structural rearrangements in the C2 domain that facilitate its interaction with lipid membranes. {ECO:0000250\|UniProtKB:P10820}.
Involvement in disease DISEASE: Hemophagocytic lymphohistiocytosis, familial, 2 (FHL2) [MIM:603553]: A rare disorder characterized by immune dysregulation with hypercytokinemia, defective function of natural killer cell, and massive infiltration of several organs by activated lymphocytes and macrophages. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, and less frequently neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits and ataxia. {ECO:0000269\|PubMed:10583959, ECO:0000269\|PubMed:11179007}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P14222
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human spleen stained with anti-perforin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic granular staining of cytotoxic T cells

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.