Immunohistochemistry (IHC) : Human tonsil stained with anti-LEF1 using peroxidase conjugate and DAB chromogen. Note nuclear staining of subset of B cells.

rabbit anti-LEF1 monoclonal antibody (ZR336) 6241

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to LEF1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: CLL/SLL
Visualization: Nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6241parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, Hematopathology, LEF1

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-LEF1 monoclonal antibody ZR336 6241

antibody
Database link: human Q9UJU2
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: CLL/SLL
Immunogen Fragment (around aa 100-200) of human LEF1 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Lymphoid enhancer-binding factor 1 (LEF-1) (T cell-specific transcription factor 1-alpha) (TCF1-alpha)
Gene names LEF1,LEF1
Protein family TCF/LEF family
Mass 44201Da
Function FUNCTION: Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540). Regulates T-cell receptor alpha enhancer function (PubMed:19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity). {ECO:0000250\|UniProtKB:P27782, ECO:0000269\|PubMed:11266540, ECO:0000269\|PubMed:19653274, ECO:0000269\|PubMed:2010090}.; FUNCTION: [Isoform 1]: Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. {ECO:0000269\|PubMed:19653274}.; FUNCTION: [Isoform 3]: Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling. {ECO:0000269\|PubMed:11326276}.; FUNCTION: [Isoform 5]: Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. {ECO:0000269\|PubMed:19653274}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding. {ECO:0000250}.
Tissues TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. {ECO:0000269\|PubMed:19653274}.
Structure SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds ALYREF/THOC4, MDFI and MDFIC. Interacts with NLK. Interacts with DAZAP2 (PubMed:19304756). {ECO:0000250\|UniProtKB:P27782, ECO:0000269\|PubMed:12192039, ECO:0000269\|PubMed:12556497, ECO:0000269\|PubMed:19304756, ECO:0000269\|PubMed:9119228, ECO:0000269\|PubMed:9488439, ECO:0000269\|PubMed:9751710}.
Post-translational modification PTM: Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway. {ECO:0000269\|PubMed:12556497}.
Domain DOMAIN: Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
Target Relevance information above includes information from UniProt accession: Q9UJU2
The UniProt Consortium

Data

Human tonsil stained with anti-LEF1 using peroxidase conjugate and DAB chromogen. Note nuclear staining of subset of B cells.

FAQ & Publications

Frequently Asked Questions

What species does the rabbit anti-LEF1 monoclonal antibody (ZR336) specifically react with?

This antibody is reactive with human tissues only.

Which applications is the rabbit anti-LEF1 monoclonal antibody (ZR336) validated for?

The antibody is suitable and tested for Immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues.

What are the recommended storage conditions for this LEF1 antibody?

For short-term storage, keep the antibody at 2-8°C. For long-term storage, it should be kept at -20°C, and freeze/thaw cycles should be avoided.

What is the host species and clonality of the anti-LEF1 antibody ZR336?

The antibody is a rabbit monoclonal antibody.

What dilutions are recommended when using the concentrated form of this LEF1 antibody for IHC?

The recommended dilution range for the concentrated antibody is 1:100 to 1:200 for immunohistochemistry.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.