Immunohistochemistry (IHC) : Human tonsil stained with anti-CD5 antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of T-cells and no stain of B-cells.

rabbit anti-CD5 monoclonal antibody (ZR228) 6101

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to CD5
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Tonsil
Visualization: Membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6101parent Category: antibody Tags: anatomy, CD5, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CD5 monoclonal antibody ZR228 6101

antibody
Database link: human P06127
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil
Immunogen Human CD5 protein fragment (around aa 269-366)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names T-cell surface glycoprotein CD5 (Lymphocyte antigen T1/Leu-1) (CD antigen CD5)
Gene names CD5,CD5 LEU1
Mass 54578Da
Function FUNCTION: Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed:1384049, PubMed:1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed:23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed:27499044). {ECO:0000250\|UniProtKB:P13379, ECO:0000269\|PubMed:1384049, ECO:0000269\|PubMed:1385158, ECO:0000269\|PubMed:23376399, ECO:0000269\|PubMed:27499044}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000250\|UniProtKB:P13379}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P13379}.
Structure SUBUNIT: Interacts with CD72/LYB-2 (PubMed:1711157). Interacts with PTPN6/SHP-1 (By similarity). Interacts with CBL (PubMed:23376399). Interacts with CD5L (By similarity). Interacts with CD3Z/CD247 (PubMed:1384049, PubMed:1385158). {ECO:0000250\|UniProtKB:P13379, ECO:0000269\|PubMed:1384049, ECO:0000269\|PubMed:1385158, ECO:0000269\|PubMed:1711157, ECO:0000269\|PubMed:23376399}.
Post-translational modification PTM: Phosphorylated on serine, threonine and tyrosine residues following TCR stimulation (PubMed:1385158). Phosphorylated by LCK on Tyr-453 and Tyr-487 upon TCR engagement. {ECO:0000250\|UniProtKB:P13379, ECO:0000269\|PubMed:1385158}.
Target Relevance information above includes information from UniProt accession: P06127
The UniProt Consortium

Data

Human tonsil stained with anti-CD5 antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of T-cells and no stain of B-cells.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.