Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with anti-CD44 using peroxidase-conjugate and DAB chromogen. Note the cell membrane staining of tumor cells

rabbit anti-CD44 monoclonal antibody (ZR184) 6095

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to CD44
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Colon carcinoma
Visualization: Cell membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6095parent Category: antibody Tags: anatomy, CD44, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CD44 monoclonal antibody ZR184 6095

antibody
Database link: human P16070
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Colon carcinoma
Immunogen Recombinant Human CD44 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names CD44 antigen (CDw44) (Epican) (Extracellular matrix receptor III) (ECMR-III) (GP90 lymphocyte homing/adhesion receptor) (HUTCH-I) (Heparan sulfate proteoglycan) (Hermes antigen) (Hyaluronate receptor) (Phagocytic glycoprotein 1) (PGP-1) (Phagocytic glycoprotein I) (PGP-I) (CD antigen CD44)
Gene names CD44,CD44 LHR MDU2 MDU3 MIC4
Mass 81538Da
Function FUNCTION: Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640). {ECO:0000269\|PubMed:15123640, ECO:0000269\|PubMed:16541107, ECO:0000269\|PubMed:18757307, ECO:0000269\|PubMed:19703720, ECO:0000269\|PubMed:22726066, ECO:0000269\|PubMed:23589287, ECO:0000269\|PubMed:7528188}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:22726066, ECO:0000269\|PubMed:23589287}; Single-pass type I membrane protein {ECO:0000255}. Cell projection, microvillus {ECO:0000250\|UniProtKB:P15379}. Secreted {ECO:0000269\|PubMed:25326458, ECO:0000269\|PubMed:36213313, ECO:0000269\|PubMed:37453717}. Note=Colocalizes with actin in membrane protrusions at wounding edges. Co-localizes with RDX, EZR and MSN in microvilli. Localizes to cholesterol-rich membrane-bound lipid raft domains. {ECO:0000250\|UniProtKB:P15379, ECO:0000269\|PubMed:23589287}.
Tissues TISSUE SPECIFICITY: Detected in fibroblasts and urine (at protein level) (PubMed:25326458, PubMed:36213313, PubMed:37453717). Detected in placenta (at protein level) (PubMed:32337544). Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. {ECO:0000269\|PubMed:25326458, ECO:0000269\|PubMed:32337544, ECO:0000269\|PubMed:36213313, ECO:0000269\|PubMed:37453717}.
Structure SUBUNIT: Interacts with PKN2 (PubMed:15123640). Interacts with TIAM1 and TIAM2 (By similarity). Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment (PubMed:14992719, PubMed:17085435, PubMed:25195884). Interacts with UNC119 (PubMed:19381274). Interacts with PDPN (via extracellular domain); this interaction is required for PDPN-mediated directional migration and regulation of lamellipodia extension/stabilization during cell spreading and migration (PubMed:20962267). Interacts with RDX, EZR and MSN (By similarity). Interacts with EGFR (PubMed:18757307, PubMed:23589287). Interacts with CD74; this complex is essential for the MIF-induced signaling cascade that results in B cell survival (By similarity). {ECO:0000250\|UniProtKB:P15379, ECO:0000269\|PubMed:14992719, ECO:0000269\|PubMed:15123640, ECO:0000269\|PubMed:17085435, ECO:0000269\|PubMed:18757307, ECO:0000269\|PubMed:19381274, ECO:0000269\|PubMed:20962267, ECO:0000269\|PubMed:23589287, ECO:0000269\|PubMed:25195884}.
Post-translational modification PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. {ECO:0000269\|PubMed:12883358}.; PTM: N-glycosylated. {ECO:0000269\|PubMed:12883358, ECO:0000269\|PubMed:16335952, ECO:0000269\|PubMed:19159218, ECO:0000269\|PubMed:22171320}.; PTM: O-glycosylated; contains chondroitin sulfate glycans which can be more or less sulfated and whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. {ECO:0000269\|PubMed:12883358, ECO:0000269\|PubMed:22171320, ECO:0000269\|PubMed:25326458}.; PTM: Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. {ECO:0000269\|PubMed:12032545, ECO:0000269\|PubMed:9580567}.
Domain DOMAIN: The lectin-like LINK domain is responsible for hyaluronan binding. {ECO:0000250}.
Target Relevance information above includes information from UniProt accession: P16070
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with anti-CD44 using peroxidase-conjugate and DAB chromogen. Note the cell membrane staining of tumor cells

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.