Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD43 antibody using peroxidase-conjugate and DAB chromogen. Note the cell membrane staining of perifollicular T-cells

rabbit anti-CD43 monoclonal antibody (ZR403) 6094

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to CD43
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Tonsil, CLL/SLL, or lymph node
Visualization: Cell membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6094parent Category: antibody Tags: anatomy, CD43, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CD43 monoclonal antibody ZR403 6094

antibody
Database link: human P16150
Tested applications IHC
Recommended dilutions As directed
Immunogen Recombinant full-length human SPN protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Leukosialin (GPL115) (Galactoglycoprotein) (GALGP) (Leukocyte sialoglycoprotein) (Sialophorin) (CD antigen CD43) [Cleaved into: CD43 cytoplasmic tail (CD43-ct) (CD43ct)]
Mass 40322Da
Function Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity). {ECO:0000250\|UniProtKB:P15702, ECO:0000269\|PubMed:18036228, ECO:0000269\|PubMed:24328034}.; [CD43 cytoplasmic tail]: Protects cells from apoptotic signals, promoting cell survival. {ECO:0000250\|UniProtKB:P15702}.
Subellular location Membrane {ECO:0000255}; Single-pass type I membrane protein {ECO:0000255}. Cell projection, microvillus {ECO:0000250\|UniProtKB:P13838}. Cell projection, uropodium {ECO:0000250\|UniProtKB:P15702}. Note=Localizes to the uropodium and microvilli via its interaction with ERM proteins (EZR, RDX and MSN). {ECO:0000250\|UniProtKB:P13838, ECO:0000250\|UniProtKB:P15702}.; [CD43 cytoplasmic tail]: Nucleus {ECO:0000269\|PubMed:15003504}. Nucleus, PML body {ECO:0000250\|UniProtKB:P15702}. Note=The sumoylated form localizes to the PML body. {ECO:0000250\|UniProtKB:P15702}.
Tissues Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Structure Interacts with SIGLEC1. {ECO:0000250\|UniProtKB:P15702}.; [CD43 cytoplasmic tail]: Monomer (By similarity). Interacts with CTNNB1 (PubMed:15003504). Interacts with RDX (via FERM domain), EZR and MSN (By similarity). {ECO:0000250\|UniProtKB:P13838, ECO:0000250\|UniProtKB:P15702, ECO:0000269\|PubMed:15003504}.
Post-translational modification Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. {ECO:0000269\|PubMed:1731338}.; Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes. {ECO:0000250\|UniProtKB:P15702}.; Has a high content of sialic acid and O-linked carbohydrate structures.; Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus. {ECO:0000269\|PubMed:15540986, ECO:0000269\|PubMed:18586676}.; [CD43 cytoplasmic tail]: Sumoylated.
Target Relevance information above includes information from UniProt accession: P16150
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD43 antibody using peroxidase-conjugate and DAB chromogen. Note the cell membrane staining of perifollicular T-cells

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.