Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD35 antibody using peroxidase-conjugated and DAB chromogen. Note cell surface staining of follicular dendritic cells

rabbit anti-CD35 monoclonal antibody (ZR58) 6089

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to CD35
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Tonsil
Visualization: Cell membrane/surface
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6089parent Category: antibody Tags: anatomy, CD35, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CD35 monoclonal antibody ZR58 6089

antibody
Database link: human P17927
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Tonsil
Immunogen Recombinant fragment (within aa650-850) of human CD35
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Complement receptor type 1 (C3b/C4b receptor) (CD antigen CD35)
Protein family Receptors of complement activation (RCA) family
Mass 223663Da
Function Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728).; (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Subellular location Membrane ; Single-pass type I membrane protein.
Tissues Present on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes and granulocytes.
Structure Interacts (via Sushi 1 and Sushi 2 domains) with complement factor C4b (PubMed:2972794, PubMed:8175757). Interacts (via Sushi 8 and Sushi 9 domains) with complement factor C3b (PubMed:2972794, PubMed:8175757). Interacts (via Sushi 24 and Sushi 25 domains) with MBL2 (PubMed:23460739, PubMed:29563915). Interacts with FCN2 (PubMed:2972794). Interacts (via Sushi 24 and Sushi 25 domains) with C1QA (PubMed:29563915, PubMed:9324355). Interacts with CR2 (PubMed:1708808).; (Microbial infection) Interacts with Epstein-Barr virus gp350.
Domain DOMAIN 42..101; /note="Sushi 1"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU00302"; DOMAIN 102..163;
Target Relevance information above includes information from UniProt accession: P17927
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD35 antibody using peroxidase-conjugated and DAB chromogen. Note cell surface staining of follicular dendritic cells

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.