Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD35 antibody using peroxidase-conjugated and DAB chromogen. Note cell surface staining of follicular dendritic cells

rabbit anti-CD35 monoclonal antibody (ZR58) 6089

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to CD35
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Tonsil
Visualization: Cell membrane/surface
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6089parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, CD35, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CD35 monoclonal antibody ZR58 6089

antibody
Database link: human P17927
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil
Immunogen Recombinant fragment (within aa650-850) of human CD35
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Complement receptor type 1 (C3b/C4b receptor) (CD antigen CD35)
Gene names CR1,CR1 C3BR
Protein family Receptors of complement activation (RCA) family
Mass 223663Da
Function FUNCTION: Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069). Also acts in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728). {ECO:0000269\|PubMed:25742728, ECO:0000269\|PubMed:2963069, ECO:0000269\|PubMed:2972794, ECO:0000269\|PubMed:8175757}.; FUNCTION: (Microbial infection) Acts as a receptor for Epstein-Barr virus. {ECO:0000269\|PubMed:23416052}.
Subellular location SUBCELLULAR LOCATION: Membrane {ECO:0000269\|PubMed:1385479}; Single-pass type I membrane protein.
Tissues TISSUE SPECIFICITY: Present on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes and granulocytes. {ECO:0000269\|PubMed:1534036, ECO:0000269\|PubMed:25742728, ECO:0000269\|PubMed:6233222}.
Structure SUBUNIT: Interacts (via Sushi 1 and Sushi 2 domains) with complement factor C4b (PubMed:2972794, PubMed:8175757). Interacts (via Sushi 8 and Sushi 9 domains) with complement factor C3b (PubMed:2972794, PubMed:8175757). Interacts (via Sushi 24 and Sushi 25 domains) with MBL2 (PubMed:23460739, PubMed:29563915). Interacts with FCN2 (PubMed:2972794). Interacts (via Sushi 24 and Sushi 25 domains) with C1QA (PubMed:29563915, PubMed:9324355). Interacts with CR2 (PubMed:1708808). {ECO:0000269\|PubMed:1708808, ECO:0000269\|PubMed:23460739, ECO:0000269\|PubMed:29563915, ECO:0000269\|PubMed:2972794, ECO:0000269\|PubMed:8175757, ECO:0000269\|PubMed:9324355}.; SUBUNIT: (Microbial infection) Interacts with Epstein-Barr virus gp350. {ECO:0000269\|PubMed:23460739}.
Target Relevance information above includes information from UniProt accession: P17927
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD35 antibody using peroxidase-conjugated and DAB chromogen. Note cell surface staining of follicular dendritic cells

FAQ & Publications

Frequently Asked Questions

What species does the rabbit anti-CD35 monoclonal antibody (ZR58) specifically react with?

This antibody specifically reacts with human CD35 protein.

For which application is the rabbit anti-CD35 monoclonal antibody (ZR58) recommended?

It is suitable for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded human tissues.

What are the storage conditions recommended for this rabbit anti-CD35 monoclonal antibody?

For short-term storage, keep the antibody at 2-8°C. For long-term storage, it should be stored at -20°C, avoiding repeated freeze/thaw cycles.

What is the immunogen used to generate the rabbit anti-CD35 monoclonal antibody (ZR58)?

The immunogen is a recombinant fragment within amino acids 650-850 of the human CD35 protein.

What is the recommended dilution range when using the concentrated form of this antibody for immunohistochemistry?

The recommended dilution for the concentrated antibody in IHC applications is between 1:100 and 1:200.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.