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Mycoplasma pneumoniae IgM ELISA kit-SERION classic ESR127M

$364.00

Summary

Virion/Serion Diagnostic Kit for research use (RUO)
Mycoplasma pneumoniae IgM ELISA Kit
Suitable for IgM detection
Ready-to-use
96 tests

SKU: ESR127M Category: Virion/Serion kits Tags: ELISA, IgM, Mycoplasma pneumoniae

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Mycoplasma pneumoniae reactive IgM
species reactivity	Mycoplasma pneumoniae
applications	ELISA
assay type	Indirect & quantitative
available sizes	96 tests

Mycoplasma pneumoniae IgM ELISA Kit ESR127M

Assay type
Indirect ELISA

Research area
Infectious Disease

Sample type
Serum, plasma, whole blood

Notes
Pretreatment of samples with RF-Absorbent (Z200) is recommended for use with IgM ELISA kits to eliminate presence of sample rheumatoid factors and possible false negative results.

Components

Break apart microtiter test strips each with antigen coated single wells	8 x 12 (96 Total)
Standard serum (ready-to-use)	2 x 2 mL
Negative control serum (ready-to-use)	2 mL
Anti-human-IgM-conjugate (ready-to-use)	13 mL
Washing solution concentrate (sufficient for 1000ml)	33.3 mL
Dilution buffer	2 x 50 mL
Stopping solution	15 mL
Substrate (ready-to-use)	13 mL
Quality control certificate with standard curve and evaluation table	1

Storage
Store at 2-8°C.

Associated products
Mycoplasma pneumoniae Antigen (BA127VS)
Mycoplasma pneumoniae P1 Antigen (BA127VSP1)
Mycoplasma pneumoniae IgA Control Serum (BC127A)
Mycoplasma pneumoniae IgG Control Serum (BC127G)
Mycoplasma pneumoniae IgM Control Serum (BC127M)
Mycoplasma pneumoniae IgA ELISA Kit (ESR127A)
Mycoplasma pneumoniae IgG ELISA Kit (ESR127G)
Mycoplasma pneumoniae IgM ELISA Kit (ESR127M)

target relevance

human antibody anti-Mycoplasma pneumoniae

Mycoplasma pneumoniae

Description
Bacterium that causes atypical pneumonia and respiratory tract infections

Structure
Mycoplasma pneumoniae is a small, self-replicating bacterium belonging to the family Mycoplasmataceae. Unlike most bacteria, M. pneumoniae lacks a cell wall and is instead surrounded by a sterol-containing cell membrane, making it naturally resistant to beta-lactam antibiotics such as penicillins and cephalosporins. The organism is highly specialized for colonization of the human respiratory tract, where it attaches to ciliated epithelial cells using a specialized attachment organelle. Mycoplasma pneumoniae is one of the smallest free-living organisms capable of independent replication and possesses a highly reduced genome reflecting its dependence on host-derived nutrients. The bacterium is transmitted through respiratory droplets and is a common cause of community-acquired respiratory infections worldwide, particularly among school-aged children, adolescents, and young adults.

Diagnosis
The diagnosis of Mycoplasma pneumoniae infection is performed through molecular, serological, and microbiological methods. Polymerase chain reaction (PCR) assays are considered the most sensitive and specific diagnostic tools and allow rapid detection of M. pneumoniae DNA from respiratory specimens. Serological assays, including ELISA-based tests, are commonly used to detect Mycoplasma-specific IgM and IgG antibodies and may assist in the diagnosis of acute or recent infection. Culture of M. pneumoniae is possible using specialized media but is rarely performed in routine clinical practice due to slow growth and technical complexity. Chest radiography may reveal diffuse or patchy infiltrates that are often disproportionate to the severity of clinical symptoms. Laboratory confirmation is particularly useful because clinical findings frequently overlap with those of viral respiratory infections.

Symptoms
Mycoplasma pneumoniae infection typically develops following an incubation period of approximately one to four weeks. Common symptoms include persistent dry cough, sore throat, fever, headache, fatigue, and malaise. Respiratory illness often begins gradually and may progress from upper respiratory tract infection to atypical pneumonia characterized by prolonged cough and diffuse pulmonary involvement. Many patients remain ambulatory despite radiographic evidence of pneumonia, leading to the term 'walking pneumonia.' Extrapulmonary manifestations may occur and can include skin rashes, hemolytic anemia, myocarditis, pericarditis, hepatitis, arthritis, and neurological complications such as encephalitis or Guillain-Barré syndrome. Although most infections are self-limiting, severe disease may occur in immunocompromised individuals and patients with underlying respiratory conditions.

Data

FAQ & Publications

Frequently Asked Questions

What type of samples is the Mycoplasma pneumoniae IgM ELISA Kit ESR127M compatible with?

The kit is suitable for use with serum, plasma, and whole blood samples.

How should the Mycoplasma pneumoniae IgM ELISA Kit ESR127M be stored to maintain its stability?

The kit should be stored at a temperature between 2 and 8 degrees Celsius.

Why is pretreatment with RF-Absorbent (Z200) recommended when using this IgM ELISA kit?

Pretreatment with RF-Absorbent Z200 is recommended to remove IgM rheumatoid factors from serum and plasma samples, which helps to avoid false negative results and improves signal specificity.

What type of assay is employed in the Mycoplasma pneumoniae IgM ELISA Kit ESR127M?

The kit utilizes an indirect and quantitative ELISA assay format for the detection of Mycoplasma pneumoniae reactive IgM antibodies.

Is the Mycoplasma pneumoniae IgM ELISA Kit ESR127M intended for clinical diagnostic use or research purposes?

This kit is intended for research use only (RUO) and is not for clinical diagnostic purposes.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
ESR127M protocol

Documents

#
EQ0045	Safety Data Sheet	QC certificate

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Mycoplasma pneumoniae IgM ELISA kit-SERION classic ESR127M

RF-Absorbent Z200 for use with IgM ELISA kits

Data

FAQ & Publications

Protocols

Documents

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Reviews

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