Immunohistochemistry (IHC) : Human tonsil stained with anti-Granzyme B antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic granular staining of cytotoxic T-cells.

mouse anti-Granzyme B monoclonal antibody (ZM66) 6204

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to Granzyme B
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2b
Control: Tonsil
Visualization: Granular cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6204parent Category: antibody Tags: anatomy, Granzyme B, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Granzyme B monoclonal antibody ZM66 6204

antibody
Database link: human P10144
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil
Immunogen Recombinant human GZMB protein fragment (around aa 73-187)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2b - Isotype Control

target relevance
Protein names Granzyme B (EC 3.4.21.79) (C11) (CTLA-1) (Cathepsin G-like 1) (CTSGL1) (Cytotoxic T-lymphocyte proteinase 2) (Lymphocyte protease) (Fragmentin-2) (Granzyme-2) (Human lymphocyte protein) (HLP) (SECT) (T-cell serine protease 1-3E)
Gene names GZMB,GZMB CGL1 CSPB CTLA1 GRB
Protein family Peptidase S1 family, Granzyme subfamily
Mass 27716Da
Function FUNCTION: Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:1985927, PubMed:3262682, PubMed:3263427). It cleaves after Asp (PubMed:1985927, PubMed:8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed:31953257, PubMed:32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed:9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity). {ECO:0000250\|UniProtKB:P04187, ECO:0000269\|PubMed:1985927, ECO:0000269\|PubMed:31953257, ECO:0000269\|PubMed:32188940, ECO:0000269\|PubMed:3262682, ECO:0000269\|PubMed:3263427, ECO:0000269\|PubMed:8258716, ECO:0000269\|PubMed:9852092}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: -Asp-\|-Xaa- >> -Asn-\|-Xaa- > -Met-\|-Xaa-, -Ser-\|-Xaa-.; EC=3.4.21.79; Evidence={ECO:0000269\|PubMed:1985927, ECO:0000269\|PubMed:32188940, ECO:0000269\|PubMed:8258716, ECO:0000269\|PubMed:9852092};
Subellular location SUBCELLULAR LOCATION: Secreted {ECO:0000269\|PubMed:20038786, ECO:0000269\|PubMed:8258716}. Cytolytic granule {ECO:0000269\|PubMed:1985927, ECO:0000269\|PubMed:24088571, ECO:0000269\|PubMed:8258716}. Note=Delivered into the target cell by perforin (PubMed:20038786). {ECO:0000269\|PubMed:20038786, ECO:0000269\|PubMed:8258716}.
Target Relevance information above includes information from UniProt accession: P10144
The UniProt Consortium

Data

Human tonsil stained with anti-Granzyme B antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic granular staining of cytotoxic T-cells.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.