ICC/IF image chicken anti Hsp60 polyclonal antibody 1416

chicken anti-Hsp60 polyclonal antibody 1416

$100.00 – $2,600.00

Antibody summary

Chicken polyclonal to Hsp60
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgY
100 µg, 25 µg, 1 mg

available sizes

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SKU: 1416parent Categories: antibody, loading control Tag: Hsp60

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

chicken anti-Hsp60 polyclonal antibody 1416

antibody
Database link: human P10809 mouse P63038 rat P63039
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 10000-20000 IF/ICC and IHC: 1: 2000-5000
Immunogen Recombinant full length human HSP60 expressed in and purified from E. coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance
Heat shock protein 60 (HSP60), also known as chaperonin 60, is a highly conserved molecular chaperone in mitochondria that assists in the folding and assembly of newly synthesized proteins, particularly under conditions of cellular stress. This antibody can be used as a loading control when run alongside proteins, particularly mitochondrail proteins, of interest with different and resolvable molecular weights and ideally in combination with antibodies of same host and when using a secondary antibody. Click for more on: loading controls and HSP60
Protein names 60 kDa heat shock protein, mitochondrial (EC 5.6.1.7) (60 kDa chaperonin) (Chaperonin 60) (CPN60) (Heat shock protein 60) (HSP-60) (Hsp60) (Heat shock protein family D member 1) (HuCHA60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein)
Gene names HSPD1,HSPD1 HSP60
Protein family Chaperonin (HSP60) family
Mass 61055Da
Function FUNCTION: Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:11422376, PubMed:1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable). {ECO:0000269\|PubMed:11422376, ECO:0000269\|PubMed:1346131, ECO:0000305\|PubMed:25918392}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.; EC=5.6.1.7; Evidence={ECO:0000305};
Subellular location SUBCELLULAR LOCATION: Mitochondrion matrix.
Structure SUBUNIT: Homoheptamer arranged in a ring structure (PubMed:11422376, PubMed:1346131, PubMed:25918392). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. Interacts with 2 heptameric Hsp10 rings to form the symmetrical football complex (PubMed:25918392). Interacts with HRAS (By similarity). Interacts with ATAD3A (PubMed:22664726). Interacts with ETFBKMT and EEF1AKMT3 (PubMed:23349634). Interacts with MFHAS1 (PubMed:24286120). {ECO:0000250\|UniProtKB:P63038, ECO:0000269\|PubMed:11422376, ECO:0000269\|PubMed:1346131, ECO:0000269\|PubMed:22664726, ECO:0000269\|PubMed:23349634, ECO:0000269\|PubMed:24286120, ECO:0000269\|PubMed:25918392}.; SUBUNIT: (Microbial infection) Interacts with hepatitis B virus/HBV protein X. {ECO:0000269\|PubMed:15120623}.; SUBUNIT: (Microbial infection) Interacts with HTLV-1 protein p40tax. {ECO:0000269\|PubMed:1731090}.
Involvement in disease DISEASE: Spastic paraplegia 13, autosomal dominant (SPG13) [MIM:605280]: A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. {ECO:0000269\|PubMed:11898127}. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: Leukodystrophy, hypomyelinating, 4 (HLD4) [MIM:612233]: A severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound intellectual disability. Death usually occurs within the first two decades of life. {ECO:0000269\|PubMed:18571143}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P10809
The UniProt Consortium

Data

Immunofluorescent analysis of of HeLa cells stained with antibody 1416 chicken pAb to HSP60, dilution 1:5,000 in green and costained with mouse mAb to actin, 7047, dilution 1:500 in red. Blue is DAPI staining of nuclear DNA. The 1416 antibody produces strong and specific staining of mitochondria, while the actin antibody labels the submembranous cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebral cortex section with chicken pAb to HSP60, 1416, dilution 1:15,000, detected in DAB (brown) following the 8718C method. Hematoxylin (blue) was used as the counterstain. HSP60 antibody specifically detects mitochondria. Mouse select image at left for larger view.

Western blot analysis of different cell lysates using chicken pAb to HSP60, 1416, dilution 1:20,000, in green. [1] protein standard (red), [2] SH-SY5Y cells, [3] HeLa cells. The strong single strong band corresponds to the HSP60 protein with an apparent SDS-PAGE molecular weight of 60kDa.

Publications

pmid	title	authors	citation
37129502	Activation of the cGAS-STING innate immune response in cells with deficient mitochondrial topoisomerase TOP1MT.	Iman Al Khatib, Jingti Deng, Yuanjiu Lei, Sylvia Torres-Odio, Gladys R Rojas, Laura E Newman, Brian K Chung, Andrew Symes, Hongliang Zhang, Shar-Yin N Huang, Yves Pommier, Aneal Khan, Gerald S Shadel, Andrew Phillip West, William T Gibson, Timothy E Shutt	Hum Mol Genet 32:2422-2440
33009713	Localization of CDR2L and CDR2 in paraneoplastic cerebellar degeneration.	Ida Herdlevaer, Torbj�rn Kr�kenes, Manja Schubert, Christian A Vedeler	Ann Clin Transl Neurol 7:2231-2242
22074436	Heat shock protein-60 and risk for cardiovascular disease.	Manfredi Rizzo, Alberto J L Macario, Everly Conway de Macario, Ioanna Gouni-Berthold, Heiner K Berthold, Giovam Battista Rini, Giovanni Zummo, Francesco Cappello	Curr Pharm Des 17:3662-8
14585136	Chaperonins are cell-signalling proteins: the unfolding biology of molecular chaperones.	J C Ranford, A R Coates, B Henderson	Expert Rev Mol Med 2:1-17
10604986	Cutting edge: heat shock protein (HSP) 60 activates the innate immune response: CD14 is an essential receptor for HSP60 activation of mononuclear cells.	A Kol, A H Lichtman, R W Finberg, P Libby, E A Kurt-Jones	J Immunol 164:13-7

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.