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chicken anti-Hsp60 polyclonal antibody 1416

$100.00$2,600.00

Antibody summary

  • Chicken polyclonal to Hsp60
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat
  • Isotype: IgY
  • 100 µg, 25 µg, 1 mg
SKU: 1416parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

chicken

isotype

IgY

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

reactivity

human, mouse, rat

available sizes

1 mg, 100 µg, 25 µg

chicken anti-Hsp60 polyclonal antibody 1416

antibody
Database link:
human P10809
mouse P63038
rat P63039
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 10000-20000 IF/ICC and IHC: 1: 2000-5000
Immunogen
Recombinant full length human HSP60 expressed in and purified from E. coli
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
polyclonal
Isotype
IgY
Compatible secondaries
goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036
goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317
goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718
goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control
Chicken polyclonal - Isotype Control
target relevance
Heat shock protein 60 (HSP60), also known as chaperonin 60, is a highly conserved molecular chaperone in mitochondria that assists in the folding and assembly of newly synthesized proteins, particularly under conditions of cellular stress. This antibody can be used as a loading control when run alongside proteins, particularly mitochondrail proteins, of interest with different and resolvable molecular weights and ideally in combination with antibodies of same host and when using a secondary antibody.

Click for more on: loading controls and HSP60
Protein names
60 kDa heat shock protein, mitochondrial (EC 5.6.1.7) (60 kDa chaperonin) (Chaperonin 60) (CPN60) (Heat shock protein 60) (HSP-60) (Hsp60) (HuCHA60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein)
Gene names
HSPD1,HSPD1 HSP60
Protein family
Chaperonin (HSP60) family
Mass
61055Da
Function
Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Catalytic activity
Reaction=ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.; EC=5.6.1.7; Evidence=;
Subellular location
Mitochondrion matrix.
Structure
Homoheptamer arranged in a ring structure (PubMed:1346131, PubMed:11422376, PubMed:25918392). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. Interacts with 2 heptameric Hsp10 rings to form the symmetrical football complex (PubMed:25918392). Interacts with HRAS (By similarity). Interacts with ATAD3A (PubMed:22664726). Interacts with ETFBKMT and EEF1AKMT3 (PubMed:23349634). Interacts with MFHAS1 (PubMed:24286120). ; (Microbial infection) Interacts with hepatitis B virus/HBV protein X. ; (Microbial infection) Interacts with HTLV-1 protein p40tax.
Involvement in disease
Spastic paraplegia 13, autosomal dominant (SPG13) [MIM:605280]: A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Note=The disease is caused by variants affecting the gene represented in this entry.; Leukodystrophy, hypomyelinating, 4 (HLD4) [MIM:612233]: A severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound intellectual disability. Death usually occurs within the first two decades of life. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P10809
The UniProt Consortium

ICC/IF-image-chicken-anti-Hsp60-polyclonal-antibody-1416
Immunofluorescent analysis of of HeLa cells stained with antibody 1416 chicken pAb to HSP60, dilution 1:5,000 in green and costained with mouse mAb to actin, 7047, dilution 1:500 in red. Blue is DAPI staining of nuclear DNA. The 1416 antibody produces strong and specific staining of mitochondria, while the actin antibody labels the submembranous cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.
IHC-image-chicken-anti-Hsp60-polyclonal-antibody-1416
Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebral cortex section with chicken pAb to HSP60, 1416, dilution 1:15,000, detected in DAB (brown) following the 8718C method. Hematoxylin (blue) was used as the counterstain. HSP60 antibody specifically detects mitochondria. Mouse select image at left for larger view.
WB-image-chicken-anti-Hsp60-polyclonal-antibody-1416
Western blot analysis of different cell lysates using chicken pAb to HSP60, 1416, dilution 1:20,000, in green. [1] protein standard (red), [2] SH-SY5Y cells, [3] HeLa cells. The strong single strong band corresponds to the HSP60 protein with an apparent SDS-PAGE molecular weight of 60kDa.

Publications

pmidtitleauthorscitation
37129502Activation of the cGAS-STING innate immune response in cells with deficient mitochondrial topoisomerase TOP1MTAl Khatib I, Deng J, Lei Y, Torres-Odio S, Rojas GR, Newman LE, Chung BK, Symes A, Zhang H, Huang SN, Pommier Y, Khan A, Shadel GS, West AP, Gibson WT, Shutt TE.Hum Mol Genet. 2023 Jul 20;32(15):2422-2440. doi: 10.1093/hmg/ddad062.
33009713Localization of CDR2L and CDR2 in paraneoplastic cerebellar degenerationHerdlevaer I, Kråkenes T, Schubert M, Vedeler CA.Ann Clin Transl Neurol. 2020 Nov;7(11):2231-2242. doi: 10.1002/acn3.51212. Epub 2020 Oct 3.
22074436Heat shock protein-60 and risk for cardiovascular diseaseRizzo M, Macario AJ, de Macario EC, Gouni-Berthold I, Berthold HK, Rini GB, Zummo G, Cappello F.Curr Pharm Des. 2011 Nov;17(33):3662-8. doi: 10.2174/138161211798220981.
14585136Chaperonins are cell-signalling proteins: the unfolding biology of molecular chaperonesRanford JC, Coates AR, Henderson B.Expert Rev Mol Med. 2000 Sep 15;2(8):1-17. doi: 10.1017/S1462399400002015.
10604986Cutting edge: heat shock protein (HSP) 60 activates the innate immune response: CD14 is an essential receptor for HSP60 activation of mononuclear cellsKol A, Lichtman AH, Finberg RW, Libby P, Kurt-Jones EA.J Immunol. 2000 Jan 1;164(1):13-7. doi: 10.4049/jimmunol.164.1.13.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

relevant to this product
Western blot
IHC
ICC
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