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respiraRNA 3 RT-PCR test Mikrogen 830507

$487.00

Summary

  • Mikrogen diagnostik RT PCR kit for research use (RUO)
  • Direct Respiratory syncytial virus (RSV), Influenza A, Influenza B detection
  • High sensitivity and specificity
  • Internal control for monitoring nucleic acid extraction
    (RNA/DNA) and real-time PCR inhibition in each reaction
  • Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
  • 96 tests
Weight 1 lbs
Dimensions 9 × 5 × 2 in
target

respiraRNA 3

species reactivity

For the detection of Influenza A and B and Respiratory Syncytial Virus (RSV)

applications

RT PCR

assay type

direct & qualitative

available sizes

96 tests

respiraRNA 3 RT-PCR test Mikrogen 830507

kit
Assay type
RT PCR
Research area
Infectious Disease
Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material
Notes
Mic (Magnetic Induction Cycler) validated
Roche LightCycler(c) 480 Instrument II validated
Roche covas z 480 Analyzer validated
Qiagen Rotor-Gene(c) Q validated
Bio-Rad CFX 96 validated
Applied Biosystems (QuantStudio TM 5 Dx) validated
Stratagene Mx3000P compatible
Components
Reaction Mix Cap color - yellow 2 x 768 µl
Positive Control Cap color - red 1 x 100 µl
Negative Control Cap color - green 1 x 100 µl
Control DNA Cap color - colorless 2 x 240 µl
Instructions for Use 1 Each
Storage
Store at -20°C.
Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance
Respiratory Syncytial Virus (RSV)
Description
Virus that causes respiratory syncytial virus (RSV) infection
Structure
Respiratory syncytial virus (RSV) is an enveloped, negative-sense, single-stranded RNA virus belonging to the genus Orthopneumovirus within the family Pneumoviridae. The virus possesses a lipid envelope containing fusion (F) and attachment (G) glycoproteins that mediate viral entry into host cells and promote the formation of multinucleated syncytia, a characteristic feature of RSV infection. RSV primarily infects epithelial cells lining the respiratory tract and is one of the leading causes of lower respiratory tract disease in infants and young children worldwide. The virus is highly contagious and spreads through respiratory droplets, direct contact, and contaminated surfaces. Reinfections are common throughout life because immunity following natural infection is incomplete.
Diagnosis
The diagnosis of RSV infection is performed through the detection of viral antigens, viral RNA, or RSV-specific antibodies. Molecular methods such as reverse transcription polymerase chain reaction (RT-PCR) are considered the most sensitive and specific diagnostic tests and are widely used in clinical laboratories. Rapid antigen detection assays and immunofluorescence tests may provide faster results but generally have lower sensitivity, particularly in adults. Serological assays, including ELISA-based methods, may be used in epidemiological studies or for the assessment of immune responses. Laboratory confirmation is particularly important in infants, elderly individuals, immunocompromised patients, and hospitalized patients with severe respiratory disease.
Symptoms
Respiratory syncytial virus is a major cause of upper and lower respiratory tract infections. Following an incubation period of approximately two to eight days, infected individuals typically develop symptoms including nasal congestion, runny nose, cough, sneezing, low-grade fever, and reduced appetite. In infants and young children, RSV frequently progresses to bronchiolitis or pneumonia, characterized by wheezing, rapid breathing, respiratory distress, and decreased oxygen saturation. Premature infants, elderly individuals, and immunocompromised patients are at increased risk of severe disease and hospitalization. Although most infections are self-limiting, RSV remains a significant cause of morbidity and mortality worldwide, particularly among vulnerable populations.

Data

No results found

FAQ & Publications

Frequently Asked Questions
Which pathogens can be detected using the respiraRNA 3 RT-PCR test Mikrogen 830507?
The respiraRNA 3 RT-PCR test Mikrogen 830507 is designed for the direct detection of Respiratory Syncytial Virus (RSV), Influenza A, and Influenza B.
Is the respiraRNA 3 RT-PCR test compatible with common real-time PCR cyclers and RNA/DNA extraction methods?
Yes, this test is compatible with most common real-time PCR cyclers, including Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P. It also works with various RNA/DNA extraction methods, such as Mikrogen alphaClean Mag RNA/DNA Kits.
Publications
pmid title authors citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU).
830507 protocol

Documents

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