TBEV/WNV RT-PCR test Mikrogen 830568

$487.00

Summary

Mikrogen diagnostik RT PCR kit for research use (RUO)
Direct West Nile Virus, TBE virus detection
High sensitivity and specificity
Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction
Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
96 tests

SKU: 830568 Category: Mikrogen kits Tags: RT PCR, Tick-Borne Encephalitis Virus, West Nile Virus

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	TBEV/WNV
species reactivity	For the detection of Tick Borne Encephalitis Virus and West Nile Virus
applications	RT PCR
assay type	direct & qualitative
available sizes	96 tests

TBEV/WNV RT-PCR test Mikrogen 830568

Assay type
RT PCR

Research area
Infectious Disease

Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material

Notes

Mic (Magnetic Induction Cycler)	validated
Roche LightCycler(c) 480 Instrument II	validated
Roche covas z 480 Analyzer	validated
Qiagen Rotor-Gene(c) Q	validated
Bio-Rad CFX 96	validated
Applied Biosystems (QuantStudio TM 5 Dx)	validated
Stratagene Mx3000P	compatible

Components

Reaction Mix	Cap color - yellow	2 x 768 µl
Positive Control	Cap color - red	1 x 100 µl
Negative Control	Cap color - green	1 x 100 µl
Control DNA	Cap color - colorless	2 x 240 µl
Instructions for Use		1 Each

Storage
Store at -20°C.

Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance

human antibody anti-Tick-Borne Encephalitis Virus (TBE/FSME)

Tick-Borne Encephalitis Virus (TBE/FSME)

Description
Virus that causes tick-borne encephalitis (TBE/FSME)

Structure
Tick-borne encephalitis virus (TBEV), also known as FSME virus (Frühsommer-Meningoenzephalitis virus), is an enveloped, positive-sense, single-stranded RNA virus belonging to the genus Flavivirus within the family Flaviviridae. The virus is closely related to other medically important flaviviruses, including West Nile virus, Yellow fever virus, Dengue virus, and Zika virus. TBEV is maintained in nature through transmission cycles involving ixodid ticks, primarily Ixodes ricinus and Ixodes persulcatus, and a variety of wild mammalian hosts. Three major subtypes are recognized: European, Siberian, and Far Eastern. Human infection typically occurs through the bite of an infected tick, although transmission may also occur through the consumption of unpasteurized milk or dairy products from infected animals. The virus is neurotropic and can invade the central nervous system, resulting in severe neurological disease.

Diagnosis
The diagnosis of tick-borne encephalitis virus infection is performed through serological and molecular methods. Detection of TBEV-specific IgM and IgG antibodies using ELISA is the primary laboratory approach and is considered highly sensitive and specific during the neurological phase of disease. Confirmation may be obtained using virus neutralization assays. Molecular detection by reverse transcription polymerase chain reaction (RT-PCR) may identify viral RNA during the early viremic phase but is often less useful after neurological symptoms develop because viremia is typically transient. Cerebrospinal fluid analysis frequently demonstrates pleocytosis and elevated protein concentrations in patients with central nervous system involvement. Accurate laboratory diagnosis is essential because clinical symptoms overlap with those of other viral and bacterial neurological infections.

Symptoms
Tick-borne encephalitis virus infection frequently follows a biphasic course. After an incubation period of approximately seven to fourteen days, the initial phase is characterized by nonspecific flu-like symptoms including fever, fatigue, headache, muscle aches, malaise, and nausea. Following a symptom-free interval, a second phase may occur in which the virus affects the central nervous system, resulting in meningitis, encephalitis, meningoencephalitis, or myelitis. Neurological manifestations may include severe headache, neck stiffness, confusion, impaired consciousness, tremors, paralysis, seizures, and cognitive dysfunction. While many infections remain asymptomatic or mild, severe disease can result in long-term neurological sequelae or death, particularly among older adults and patients infected with more virulent viral subtypes.

Data

No results found

FAQ & Publications

Frequently Asked Questions

What pathogens can the TBEV/WNV RT-PCR test Mikrogen 830568 detect?

This RT-PCR test is designed for the direct detection of Tick-Borne Encephalitis Virus (TBEV) and West Nile Virus (WNV).

Which sample types are compatible with this TBEV/WNV RT-PCR test?

The test can be applied to various sample types including whole blood, serum, plasma, urine, tissue, stool, food and environmental samples, or RNA/DNA extracted from ticks or carrier material.

Is the TBEV/WNV RT-PCR test compatible with common real-time PCR cyclers?

Yes, the test has been validated on multiple real-time PCR cyclers such as Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P, and is compatible with other common instruments as well.

How should the TBEV/WNV RT-PCR test kit be stored to maintain stability?

The kit components should be stored at -20°C to ensure stability and proper performance.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 830568 protocol

Documents

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TBEV/WNV RT-PCR test Mikrogen 830568

Data

FAQ & Publications

Protocols

Documents

Reviews

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