Tropical Fever 1 RT-PCR test Mikrogen 830563

$487.00

Summary

Mikrogen diagnostik RT PCR kit for research use (RUO)
Direct Chikungunya Virus, Dengue fever virus, Zikavirus, Yellow fever virus detection
High sensitivity and specificity
Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction
Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
96 tests

SKU: 830563 Category: Mikrogen kits Tags: Chikungunya Virus, Dengue Virus, RT PCR, Yellow fever virus, Zika Virus

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Tropical Fever 1
species reactivity	Detection of Zika virus, chikungunya virus, dengue virus 1-4 and yellow fever virus
applications	RT PCR
assay type	direct & qualitative
available sizes	96 tests

Tropical Fever 1 RT-PCR test Mikrogen 830563

Assay type
RT PCR

Research area
Infectious Disease

Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material

Notes

Mic (Magnetic Induction Cycler)	validated
Roche LightCycler(c) 480 Instrument II	validated
Roche covas z 480 Analyzer	validated
Qiagen Rotor-Gene(c) Q	validated
Bio-Rad CFX 96	validated
Applied Biosystems (QuantStudio TM 5 Dx)	validated
Stratagene Mx3000P	compatible

Components

Reaction Mix	Cap color - yellow	2 x 768 µl
Positive Control	Cap color - red	1 x 100 µl
Negative Control	Cap color - green	1 x 100 µl
Control DNA	Cap color - colorless	2 x 240 µl
Instructions for Use		1 Each

Storage
Store at -20°C.

Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance

human antibody anti-Chikungunyavirus

Chikungunyavirus

Description
Chikungunya virus - Virus that causes chikungunya fever

Structure
Chikungunya virus (CHIKV) is an enveloped, positive-sense, single-stranded RNA virus belonging to the genus Alphavirus within the family Togaviridae. The virus is transmitted primarily by Aedes mosquitoes, particularly Aedes aegypti and Aedes albopictus, which are also vectors for dengue and Zika viruses. Chikungunya virus is maintained in transmission cycles involving humans and mosquitoes in urban settings and may also circulate in sylvatic cycles involving non-human primates and other vertebrate hosts. The viral particle contains a lipid envelope with glycoproteins that facilitate attachment and entry into host cells. Since its identification in Tanzania in 1952, chikungunya virus has caused numerous outbreaks across Africa, Asia, Europe, and the Americas. The virus is notable for causing debilitating joint pain that may persist for months or years after acute infection.

Diagnosis
The diagnosis of chikungunya virus infection is performed through molecular and serological methods. During the acute phase of illness, reverse transcription polymerase chain reaction (RT-PCR) is the preferred diagnostic method because it allows direct detection of viral RNA in blood samples. As viremia declines, serological assays including ELISA and immunofluorescence tests are used to detect chikungunya-specific IgM and IgG antibodies. Virus neutralization assays may be employed for confirmatory testing in specialized laboratories. Laboratory findings often include lymphopenia, thrombocytopenia, and elevated inflammatory markers. Because the clinical presentation overlaps with dengue, Zika, and other arboviral infections, laboratory confirmation is important for accurate diagnosis.

Symptoms
Chikungunya fever typically develops following an incubation period of approximately two to twelve days after the bite of an infected mosquito. The disease is characterized by sudden onset of high fever, severe joint pain, headache, muscle aches, fatigue, and rash. Joint pain most commonly affects the hands, wrists, ankles, feet, and larger joints and may be severe enough to limit mobility. While the acute illness generally resolves within one to two weeks, many patients experience persistent or recurrent arthralgia, arthritis, and fatigue that can last for months or even years. Severe complications are uncommon but may include neurological disease, myocarditis, hepatitis, ocular inflammation, and, rarely, death, particularly in elderly individuals and patients with underlying medical conditions.

Data

No results found

FAQ & Publications

Frequently Asked Questions

What pathogens can the Tropical Fever 1 RT-PCR test Mikrogen 830563 detect?

The Tropical Fever 1 RT-PCR test Mikrogen 830563 is designed for direct detection of Zika virus, chikungunya virus, dengue virus (serotypes 1-4), and yellow fever virus.

Which real-time PCR instruments have been validated for use with the Tropical Fever 1 RT-PCR test Mikrogen 830563?

This test has been validated for use with several real-time PCR cyclers including the Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 830563 protocol

Documents

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Tropical Fever 1 RT-PCR test Mikrogen 830563

Data

FAQ & Publications

Protocols

Documents

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