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Coronavirus Panel RT-PCR test Mikrogen 50142

$487.00

Summary

  • Mikrogen diagnostik RT PCR kit for research use (RUO)
  • Direct SARS-CoV-2, Coronavirus, MERS-CoV detection
  • High sensitivity and specificity
  • Internal control for monitoring nucleic acid extraction
    (RNA/DNA) and real-time PCR inhibition in each reaction
  • Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
  • 96 tests
SKU: 50142 Category: Tag:
Weight 1 lbs
Dimensions 9 × 5 × 2 in
target

Coronavirus

species reactivity

For detection of Middle East Respiratory Syndrome Coronavirus (MERS CoV, SARS coronaviruses (incl. SARS-CoV-2 / COVID 19), human coronaviruses (NL63, OC43, 229E, HKU).

applications

RT PCR

assay type

direct & qualitative

available sizes

96 tests

Coronavirus Panel RT-PCR test Mikrogen 50142

kit
Assay type
RT PCR
Research area
Infectious Disease
Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material
Notes
Mic (Magnetic Induction Cycler) validated
Roche LightCycler(c) 480 Instrument II validated
Roche covas z 480 Analyzer validated
Qiagen Rotor-Gene(c) Q validated
Bio-Rad CFX 96 validated
Applied Biosystems (QuantStudio TM 5 Dx) validated
Stratagene Mx3000P compatible
Components
Reaction Mix Cap color - yellow 2 x 768 µl
Positive Control Cap color - red 1 x 100 µl
Negative Control Cap color - green 1 x 100 µl
Control DNA Cap color - colorless 2 x 240 µl
Instructions for Use 1 Each
Storage
Store at -20°C.
Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance
SARS-CoV-2
Description
Virus that causes Coronavirus Disease 2019 (COVID-19)
Structure
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus belonging to the genus Betacoronavirus within the family Coronaviridae. The viral genome is approximately 30 kilobases in length, making it one of the largest known RNA virus genomes. The virus is characterized by prominent spike (S) glycoproteins projecting from the viral envelope, which mediate attachment to host cells through the angiotensin-converting enzyme 2 (ACE2) receptor. SARS-CoV-2 primarily infects epithelial cells of the respiratory tract but can affect multiple organ systems including the cardiovascular, gastrointestinal, neurological, and renal systems. Since its emergence in late 2019, the virus has evolved into numerous variants through genetic mutation and selection, resulting in changes in transmissibility, immune evasion, and disease severity.
Diagnosis
The diagnosis of SARS-CoV-2 infection is performed through the detection of viral RNA, viral antigens, or virus-specific antibodies. Reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for the detection of viral RNA in respiratory specimens and provides high sensitivity and specificity. Rapid antigen tests are widely used for point-of-care diagnosis and screening, although they are generally less sensitive than molecular methods. Serological assays, including ELISA-based tests, may be used to detect antibodies generated following infection or vaccination and are useful for epidemiological studies and assessment of immune responses. Laboratory findings in severe cases may include elevated inflammatory markers, lymphopenia, and abnormalities in coagulation parameters.
Symptoms
SARS-CoV-2 is primarily transmitted through respiratory droplets, aerosols, and direct contact with contaminated secretions. Following an incubation period of approximately two to fourteen days, infected individuals may develop fever, cough, fatigue, headache, sore throat, nasal congestion, myalgia, and loss of taste or smell. Additional manifestations may include gastrointestinal symptoms such as nausea, vomiting, and diarrhea. Disease severity ranges from asymptomatic infection to severe pneumonia, acute respiratory distress syndrome (ARDS), multi-organ failure, and death. Elderly individuals and patients with underlying medical conditions are at increased risk of severe disease. Persistent symptoms following acute infection, commonly referred to as post-COVID condition or Long COVID, may affect multiple organ systems and persist for weeks or months after the initial illness.

Data

No results found

FAQ & Publications

Frequently Asked Questions
Which coronavirus species can be detected using the Coronavirus Panel RT-PCR test Mikrogen 50142?
The test detects Middle East Respiratory Syndrome Coronavirus (MERS-CoV), SARS coronaviruses including SARS-CoV-2 (COVID-19), and human coronaviruses NL63, OC43, 229E, and HKU.
What sample types are compatible with the Coronavirus Panel RT-PCR test Mikrogen 50142?
This RT-PCR test can be used with various sample types including whole blood, serum, plasma, urine, tissue, stool, as well as food and environmental samples or carrier material.
Is the Coronavirus Panel RT-PCR test Mikrogen 50142 compatible with common real-time PCR cyclers and nucleic acid extraction methods?
Yes, the kit is compatible with most common real-time PCR cyclers such as Roche LightCycler 480 Instrument II, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, and Applied Biosystems QuantStudio 5 Dx, and works with various RNA/DNA extraction methods including Mikrogen alphaClean Mag RNA/DNA Kits.
What are the storage conditions recommended for the Coronavirus Panel RT-PCR test Mikrogen 50142?
The kit components should be stored at -20°C to maintain stability and performance.
Publications
pmid title authors citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU).
50142 protocol

Documents

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