Borrelia_Rickettsia RT-PCR test Mikrogen 830576

$487.00

Summary

Mikrogen diagnostik RT PCR kit for research use (RUO)
Direct Borrelia burgdorferi, Rickettsia spp. detection
High sensitivity and specificity
Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction
Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
96 tests

SKU: 830576 Category: Mikrogen kits Tags: Borrelia, Rickettsia, RT PCR

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Borrelia_Rickettsia
species reactivity	For the detection of Borrelia burgdorferi and Rickettsia
applications	RT PCR
assay type	direct & qualitative
available sizes	96 tests

Borrelia_Rickettsia RT-PCR test Mikrogen 830576

Assay type
RT PCR

Research area
Infectious Disease

Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material

Notes

Mic (Magnetic Induction Cycler)	validated
Roche LightCycler(c) 480 Instrument II	validated
Roche covas z 480 Analyzer	validated
Qiagen Rotor-Gene(c) Q	validated
Bio-Rad CFX 96	validated
Applied Biosystems (QuantStudio TM 5 Dx)	validated
Stratagene Mx3000P	compatible

Components

Reaction Mix	Cap color - yellow	2 x 768 µl
Positive Control	Cap color - red	1 x 100 µl
Negative Control	Cap color - green	1 x 100 µl
Control DNA	Cap color - colorless	2 x 240 µl
Instructions for Use		1 Each

Storage
Store at -20°C.

Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance

human antibody anti-Borrelia

Borrelia

Description
Bacterial genus that causes Lyme disease and relapsing fever

Structure
Borrelia is a genus of spiral-shaped bacteria belonging to the family Spirochaetaceae. Members of the genus are Gram-negative, highly motile spirochetes characterized by a flexible helical morphology and periplasmic flagella located between the outer membrane and the peptidoglycan layer. These endoflagella enable corkscrew-like movement through tissues and viscous environments. Borrelia species are maintained in nature through complex transmission cycles involving arthropod vectors such as ticks and lice and a variety of vertebrate reservoir hosts. The genus includes pathogens responsible for Lyme borreliosis, primarily the Borrelia burgdorferi sensu lato complex, and relapsing fever caused by several other Borrelia species.

Diagnosis
The diagnosis of Borrelia infection is performed through a combination of clinical assessment, serological testing, and molecular methods. For Lyme disease, laboratory diagnosis typically involves a two-tier testing strategy consisting of an ELISA or immunoassay followed by a confirmatory immunoblot when appropriate. Detection of Borrelia-specific IgM and IgG antibodies can support the diagnosis, although antibody responses may not be detectable during the earliest stages of infection. Polymerase chain reaction (PCR) assays may be used to detect Borrelia DNA in selected clinical specimens, particularly synovial fluid, cerebrospinal fluid, or skin biopsies. Culture of Borrelia organisms is possible but technically demanding and not routinely performed in clinical laboratories.

Symptoms
Borrelia infections present with a wide spectrum of clinical manifestations depending on the infecting species and stage of disease. Lyme borreliosis commonly begins with erythema migrans, an expanding skin lesion that develops days to weeks after a tick bite and may be accompanied by fever, fatigue, headache, myalgia, and arthralgia. Untreated infection can progress to involve the nervous system, heart, and joints, resulting in neuroborreliosis, carditis, or Lyme arthritis. Relapsing fever Borrelia species cause recurrent episodes of high fever, chills, headache, muscle pain, and malaise separated by symptom-free intervals. Severe complications may occur if infections are left untreated.

Data

No results found

FAQ & Publications

Frequently Asked Questions

Which sample types are compatible with the Borrelia_Rickettsia RT-PCR test Mikrogen 830576?

This RT-PCR test is compatible with various sample types including whole blood, serum, plasma, urine, tissue, stool, as well as food and environmental samples or carrier material.

Is the Borrelia_Rickettsia RT-PCR test Mikrogen 830576 validated on common real-time PCR cyclers?

Yes, the kit has been validated for use with several real-time PCR cyclers including the Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 830576 protocol

Documents

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Borrelia_Rickettsia RT-PCR test Mikrogen 830576

Data

FAQ & Publications

Protocols

Documents

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Reviews

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