PanLegionella RT-PCR test Mikrogen 830535

$487.00

Summary

Mikrogen diagnostik RT PCR kit for research use (RUO)
Direct Legionella spec. detection
High sensitivity and specificity
Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction
Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
96 tests

SKU: 830535 Category: Mikrogen kits Tags: Legionella, RT PCR

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	PanLegionella
species reactivity	For the detection of Legionella spec.
applications	RT PCR
assay type	direct & qualitative
available sizes	96 tests

PanLegionella RT-PCR test Mikrogen 830535

Assay type
RT PCR

Research area
Infectious Disease

Sample type
whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material

Notes

Mic (Magnetic Induction Cycler)	validated
Roche LightCycler(c) 480 Instrument II	validated
Roche covas z 480 Analyzer	validated
Qiagen Rotor-Gene(c) Q	validated
Bio-Rad CFX 96	validated
Applied Biosystems (QuantStudio TM 5 Dx)	validated
Stratagene Mx3000P	compatible

Components

Reaction Mix	Cap color - yellow	2 x 768 µl
Positive Control	Cap color - red	1 x 100 µl
Negative Control	Cap color - green	1 x 100 µl
Control DNA	Cap color - colorless	2 x 240 µl
Instructions for Use		1 Each

Storage
Store at -20°C.

Additional information

Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections Applicable to human starting material as well as RNA/DNA from the tick

Complete PCR kits with ready-to-use reagents

Compatible with common real-time PCR cyclers Compatible with various RNA/DNA extraction methods (e.g. Mikrogen alphaClean Mag RNA/DNA Kits)

target relevance

human antibody anti-Legionella pneumophila

Legionella pneumophila

Description
Bacterium that causes Legionnaires' disease and Pontiac fever

Structure
Legionella pneumophila is a Gram-negative, aerobic, rod-shaped bacterium belonging to the family Legionellaceae. The organism is naturally found in freshwater environments, including lakes, rivers, and streams, where it survives and replicates within free-living amoebae and other protozoa. In man-made water systems such as cooling towers, hot water tanks, plumbing systems, decorative fountains, and whirlpool spas, Legionella can proliferate under favorable conditions and form biofilms that enhance survival and persistence. L. pneumophila is a facultative intracellular pathogen that infects and replicates within alveolar macrophages and other host cells by preventing phagosome-lysosome fusion. Serogroup 1 is responsible for the majority of human infections. Legionella pneumophila is the primary causative agent of Legionnaires' disease, a severe form of pneumonia, and Pontiac fever, a milder self-limiting illness.

Diagnosis
The diagnosis of Legionella pneumophila infection is performed through microbiological, molecular, and antigen detection methods. Detection of Legionella urinary antigen, particularly for L. pneumophila serogroup 1, is widely used due to its rapidity and high specificity. Polymerase chain reaction (PCR) assays performed on respiratory specimens provide highly sensitive detection and may identify a broader range of Legionella species and serogroups. Culture of respiratory samples on specialized buffered charcoal yeast extract (BCYE) agar remains the definitive diagnostic method and allows epidemiological comparison of clinical and environmental isolates. Serological testing may be used for retrospective diagnosis but is less useful for acute clinical management. Early diagnosis is important because prompt antimicrobial therapy significantly improves outcomes.

Symptoms
Legionella pneumophila infection most commonly occurs following inhalation of contaminated aerosols generated from water systems. After an incubation period of approximately two to ten days, Legionnaires' disease typically presents with high fever, chills, cough, shortness of breath, fatigue, headache, muscle aches, and malaise. Gastrointestinal symptoms such as diarrhea, nausea, vomiting, and abdominal pain are common and may help distinguish Legionnaires' disease from other causes of pneumonia. Neurological manifestations including confusion, disorientation, and altered mental status may also occur. Severe infections can progress to respiratory failure, septic shock, multi-organ dysfunction, and death, particularly in elderly individuals, smokers, immunocompromised patients, and those with chronic underlying diseases. Pontiac fever presents as a milder influenza-like illness without pneumonia and generally resolves spontaneously within several days.

Data

No results found

FAQ & Publications

Frequently Asked Questions

What types of samples can be tested using the PanLegionella RT-PCR test Mikrogen 830535?

The PanLegionella RT-PCR test Mikrogen 830535 can be used to detect Legionella species in various sample types including whole blood, serum, plasma, urine, tissue, stool, food, environmental samples, and carrier material.

Is the PanLegionella RT-PCR test compatible with common real-time PCR cyclers and RNA/DNA extraction methods?

Yes, this test kit is compatible with most common real-time PCR cyclers such as Roche LightCycler 480 Instrument II, Roche cobas z 480 Analyzer, Qiagen Rotor-Gene Q, Bio-Rad CFX 96, Applied Biosystems QuantStudio 5 Dx, and Stratagene Mx3000P. It is also compatible with various RNA/DNA extraction methods including Mikrogen alphaClean Mag RNA/DNA Kits.

How should the PanLegionella RT-PCR test Mikrogen 830535 be stored to maintain reagent stability?

The kit should be stored at -20°C to ensure the stability and integrity of the reagents.

What internal controls does the PanLegionella RT-PCR test include to ensure assay reliability?

The test includes an internal control in each reaction to monitor nucleic acid extraction (RNA/DNA) efficiency and to detect real-time PCR inhibition, ensuring high sensitivity and specificity in direct Legionella species detection.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 830535 protocol

Documents

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PanLegionella RT-PCR test Mikrogen 830535

Data

FAQ & Publications

Protocols

Documents

Reviews

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