Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded prostate adenocarcinoma stained with anti-NKX3.1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

rabbit anti-NKX3.1 monoclonal antibody (ZR222) 6292

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to NKX3.1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Prostate
Visualization: Nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6292parent Category: antibody Tags: anatomy, monoclonal, NKX3.1, Urinary Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-NKX3.1 monoclonal antibody ZR222 6292

antibody
Database link: human Q99801
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Prostate
Immunogen Recombinant fragment (around aa 92-224) of human NKX3.1 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Homeobox protein Nkx-3.1 (Homeobox protein NK-3 homolog A)
Protein family NK-3 homeobox family
Mass 26350Da
Function Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Subellular location Nucleus .
Tissues Highly expressed in the prostate and, at a lower level, in the testis.
Structure Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF (PubMed:11809674). Interacts with WDR77 (PubMed:12972618). Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation (PubMed:18077445). Interacts with FEM1B (By similarity).
Post-translational modification PTM: Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.
Target Relevance information above includes information from UniProt accession: Q99801
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded prostate adenocarcinoma stained with anti-NKX3.1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.