Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded bone marrow stained with anti-CD71 antibody using peroxidase-conjugated and DAB chromogen. Note the cytoplasmic staining of erythroid precursors

rabbit anti-CD71 monoclonal antibody (ZR422) 6110

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to CD71
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Bone marrow
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6110parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, CD71, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CD71 monoclonal antibody ZR422 6110

antibody
Database link: human P02786
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Bone marrow
Immunogen Recombinant fragment of human TFRC receptor
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Transferrin receptor protein 1 (TR) (TfR) (TfR1) (Trfr) (T9) (p90) (CD antigen CD71) [Cleaved into: Transferrin receptor protein 1, serum form (sTfR)]
Gene names TFRC,TFRC
Protein family Peptidase M28 family, M28B subfamily
Mass 84871Da
Function FUNCTION: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738). Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738). When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738). Mediates uptake of NICOL1 into fibroblasts where it may regulate extracellular matrix production (By similarity). {ECO:0000250\|UniProtKB:Q62351, ECO:0000269\|PubMed:26214738, ECO:0000269\|PubMed:26642240, ECO:0000269\|PubMed:3568132}.; FUNCTION: (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. {ECO:0000269\|PubMed:17287727, ECO:0000269\|PubMed:18268337}.; FUNCTION: (Microbial infection) Acts as a host entry factor for rabies virus that hijacks the endocytosis of TFRC to enter cells. {ECO:0000269\|PubMed:36779762, ECO:0000269\|PubMed:36779763}.; FUNCTION: (Microbial infection) Acts as a host entry factor for SARS-CoV, MERS-CoV and SARS-CoV-2 viruses that hijack the endocytosis of TFRC to enter cells. {ECO:0000269\|PubMed:36779762}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:17081065}; Single-pass type II membrane protein {ECO:0000269\|PubMed:17081065}. Melanosome {ECO:0000269\|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. {ECO:0000269\|PubMed:17081065}.; SUBCELLULAR LOCATION: [Transferrin receptor protein 1, serum form]: Secreted {ECO:0000269\|PubMed:17081065}.
Structure SUBUNIT: Homodimer; disulfide-linked. Binds one transferrin or HFE molecule per subunit. Binds the HLA class II histocompatibility antigen, DR1. Interacts with SH3BP3. Interacts with STEAP3; facilitates TFRC endocytosis in erythroid precursor cells (PubMed:26642240). Interacts with GRM2 (PubMed:36779763). {ECO:0000269\|PubMed:16325581, ECO:0000269\|PubMed:26642240, ECO:0000269\|PubMed:36779763}.; SUBUNIT: (Microbial infection) Interacts with Guanarito, Junin and Machupo arenavirus glycoprotein complex (PubMed:17287727, PubMed:18268337). {ECO:0000269\|PubMed:17287727, ECO:0000269\|PubMed:18268337}.; SUBUNIT: (Microbial infection) Interacts with rabies virus protein G. {ECO:0000269\|PubMed:36779762, ECO:0000269\|PubMed:36779763}.; SUBUNIT: (Microbial infection) Interacts with SARS-CoV-2 spike protein S. {ECO:0000269\|PubMed:36779762, ECO:0000269\|PubMed:36779763}.
Post-translational modification PTM: Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation (PubMed:26214738). Stearoylation does not affect iron uptake (PubMed:26214738). {ECO:0000269\|PubMed:26214738}.; PTM: N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. {ECO:0000269\|PubMed:1421756, ECO:0000269\|PubMed:1421757, ECO:0000269\|PubMed:16335952, ECO:0000269\|PubMed:19159218, ECO:0000269\|PubMed:19349973, ECO:0000269\|PubMed:3582362}.; PTM: Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).; PTM: Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. {ECO:0000269\|PubMed:3582362}.
Involvement in disease DISEASE: Immunodeficiency 46 (IMD46) [MIM:616740]: An autosomal recessive primary immunodeficiency disorder characterized by early-onset chronic diarrhea, recurrent infections, hypo- or agammaglobulinemia, normal lymphocyte counts, intermittent neutropenia, and intermittent thrombocytopenia. {ECO:0000269\|PubMed:26642240}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P02786
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded bone marrow stained with anti-CD71 antibody using peroxidase-conjugated and DAB chromogen. Note the cytoplasmic staining of erythroid precursors

FAQ & Publications

Frequently Asked Questions

What species does the rabbit anti-CD71 monoclonal antibody (ZR422) specifically react with, and in which application has it been validated?

This rabbit anti-CD71 monoclonal antibody (ZR422) specifically reacts with human species and has been validated for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues.

How should the rabbit anti-CD71 monoclonal antibody (ZR422) be stored to maintain its stability, and what are the available concentrations for this antibody?

The antibody should be stored at 2-8°C for short-term use and at -20°C for long-term storage, avoiding freeze/thaw cycles. It is available in concentrated forms of 0.1 mL, 0.5 mL, and 1.0 mL, as well as a 7 mL prediluted format.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.