Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human lymph node stained with anti-CD20 antibody using peroxidase-conjugate and DAB chromogen. Note cell surface staining of B-cells.

rabbit anti-CD20 monoclonal antibody (ZR243) 6071

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to CD20
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Tonsil, lymph node
Visualization: Cell membrane/cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6071parent Category: antibody Tags: anatomy, CD20, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CD20 monoclonal antibody ZR243 6071

antibody
Database link: human P11836
Tested applications IHC
Recommended dilutions Concentrated 1:100-300
Application Notes Positive control: Tonsil, lymph node
Immunogen Recombinant fragment (around aa 197-297) of human MS4A1 protein (exact sequence is proprietary)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names B-lymphocyte antigen CD20 (B-lymphocyte surface antigen B1) (Bp35) (Leukocyte surface antigen Leu-16) (Membrane-spanning 4-domains subfamily A member 1) (CD antigen CD20)
Gene names MS4A1,MS4A1 CD20
Protein family MS4A family
Mass 33077Da
Function FUNCTION: B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739). {ECO:0000269\|PubMed:12920111, ECO:0000269\|PubMed:18474602, ECO:0000269\|PubMed:3925015, ECO:0000269\|PubMed:7684739}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:12920111, ECO:0000269\|PubMed:22615937}; Multi-pass membrane protein {ECO:0000269\|PubMed:22615937}. Cell membrane {ECO:0000269\|PubMed:22615937}; Lipid-anchor {ECO:0000269\|PubMed:22615937}. Note=Constitutively associated with membrane rafts. {ECO:0000269\|PubMed:12920111}.
Tissues TISSUE SPECIFICITY: Expressed on B-cells.
Structure SUBUNIT: Forms homotetramers (PubMed:18474602, PubMed:7684739). Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR (PubMed:18474602). {ECO:0000269\|PubMed:18474602, ECO:0000269\|PubMed:7684739}.
Post-translational modification PTM: Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate. {ECO:0000269\|PubMed:2472394}.
Involvement in disease DISEASE: Immunodeficiency, common variable, 5 (CVID5) [MIM:613495]: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. {ECO:0000269\|PubMed:20038800}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P11836
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human lymph node stained with anti-CD20 antibody using peroxidase-conjugate and DAB chromogen. Note cell surface staining of B-cells.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.