Immunohistochemistry (IHC) : Human tonsil stained with anti-CD123 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of plasmacytoid dendritic cells.

rabbit anti-CD123 monoclonal antibody (ZR425) 6058

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to CD123
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Lymph node or thymus
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6058parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, CD123, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CD123 monoclonal antibody ZR425 6058

antibody
Database link: human O75794
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Lymph node or thymus
Immunogen Recombinant fragment of human IL3RA protein (around aa 26-171)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Homo sapiens CDC123 Translation initiation factor eIF2 assembly protein
Protein names Translation initiation factor eIF2 assembly protein
Alternative names Cell division cycle protein 123 homolog, HT-1080, PZ32
Gene names CDC123
Protein family Belongs to the CDC123 family
Function ATP-dependent protein-folding chaperone for the eIF2 complex (PubMed:35031321, PubMed:37507029). Binds to the gamma subunit of the eIF2 complex which allows the subunit to assemble with the alpha and beta subunits (By similarity)
Subcellular location Cytoplasm
Structure Interacts with the eIF2 complex gamma subunit EIF2S3 (via C-terminus); the interaction is direct (PubMed:35031321, PubMed:37507029). Interacts with the eIF2 complex alpha subunit EIF2S1 (PubMed:31836389, PubMed:35031321). Interacts with the eIF2 complex beta subunit EIF2S2 (PubMed:31836389, PubMed:35031321)
Keywords 3D-structure, ATP-binding, Chaperone, Cytoplasm, Magnesium, Metal-binding, Nucleotide-binding, Phosphoprotein, Proteomics identification, Reference proteome
Sequence MKKEHVLHCQFSAWYPFFRGVTIKSVILPLPQNVKDYLLDDGTLVVSGRDDPPTHSQPDS DDEAEEIQWSDDENTATLTAPEFPEFATKVQEAINSLGGSVFPKLNWSAPRDAYWIAMNS SLKCKTLSDIFLLFKSSDFITRDFTQPFIHCTDDSPDPCIEYELVLRKWCELIPGAEFRC FVKENKLIGISQRDYTQYYDHISKQKEEIRRCIQDFFKKHIQYKFLDEDFVFDIYRDSRG KVWLIDFNPFGEVTDSLLFTWEELISENNLNGDFSEVDAQEQDSPAFRCTNSEVTVQPSP YLSYRLPKDFVDLSTGEDAHKLIDFLKLKRNQQEDD
UniProt accession: O75794

Data

Human tonsil stained with anti-CD123 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of plasmacytoid dendritic cells.

FAQ & Publications

Frequently Asked Questions

What species does the rabbit anti-CD123 monoclonal antibody (ZR425) specifically react with?

This antibody specifically reacts with human CD123.

Which application is this anti-CD123 antibody validated for?

The rabbit anti-CD123 monoclonal antibody (ZR425) is validated for Immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues.

What are the recommended storage conditions for this antibody to maintain stability?

For short-term storage, keep the antibody at 2-8°C, and for long-term storage, it should be kept at -20°C. Avoid repeated freeze-thaw cycles to preserve antibody integrity.

What is the clonality and host species of this anti-CD123 antibody?

This antibody is a rabbit monoclonal antibody, indicating it is produced from a single clone and raised in rabbit.

What is the recommended dilution range for the concentrated form of this antibody in IHC applications?

The recommended dilution for the concentrated antibody in IHC is between 1:100 and 1:200.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.