Immunohistochemistry (IHC) : Human type B1 thymoma stained with anti-TdT antibody. Note the immature T-cells are positive.

mouse anti-TdT monoclonal antibody (ZM51) 6378

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to TdT
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Thymus
Visualization: Nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6378parent Category: antibody Tags: anatomy, Hematopathology, TdT

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-TdT monoclonal antibody ZM51 6378

antibody
Database link: human P04053
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Thymus
Immunogen Recombinant fragment (around aa 52-192) of human DNTT protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names DNA nucleotidylexotransferase (EC 2.7.7.31) (Terminal addition enzyme) (Terminal deoxynucleotidyltransferase) (Terminal transferase)
Gene names DNTT,DNTT TDT
Protein family DNA polymerase type-X family
Mass 58536Da
Function FUNCTION: Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. {ECO:0000250\|UniProtKB:P09838}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate; Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112; EC=2.7.7.31; Evidence={ECO:0000269\|PubMed:11473582, ECO:0000269\|PubMed:16371131, ECO:0000269\|PubMed:8163485};
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269\|PubMed:16371131}.
Structure SUBUNIT: Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA. Interacts with TRERF1 (PubMed:16371131). {ECO:0000269\|PubMed:11473582, ECO:0000269\|PubMed:12786946, ECO:0000269\|PubMed:12960389, ECO:0000269\|PubMed:16371131}.
Target Relevance information above includes information from UniProt accession: P04053
The UniProt Consortium

Data

Human type B1 thymoma stained with anti-TdT antibody. Note the immature T-cells are positive.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.