Immunohistochemistry (IHC) : Human tonsil stained with anti-Kappa light chain antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of plasma cells.

mouse anti-Kappa monoclonal antibody (ZM81) 6235

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to Kappa
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Tonsil or lymph node
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6235parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, Hematopathology, Kappa

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Kappa monoclonal antibody ZM81 6235

antibody
Database link: human P01601
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil or lymph node
Immunogen Recombinant full-length human IGKC protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Immunoglobulin kappa variable 1D-16 (Ig kappa chain V-I region HK146) (Ig kappa chain V-I region HK189)
Gene names IGKV1D-16,IGKV1D-16
Mass 12730Da
Function FUNCTION: V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). {ECO:0000303\|PubMed:17576170, ECO:0000303\|PubMed:20176268, ECO:0000303\|PubMed:22158414, ECO:0000303\|PubMed:24600447}.
Subellular location SUBCELLULAR LOCATION: Secreted {ECO:0000303\|PubMed:20176268, ECO:0000303\|PubMed:22158414}. Cell membrane {ECO:0000303\|PubMed:20176268, ECO:0000303\|PubMed:22158414}.
Structure SUBUNIT: Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked. {ECO:0000303\|PubMed:20176268}.
Target Relevance information above includes information from UniProt accession: P01601
The UniProt Consortium

Data

Human tonsil stained with anti-Kappa light chain antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of plasma cells.

FAQ & Publications

Frequently Asked Questions

What species does the mouse anti-Kappa monoclonal antibody (ZM81) specifically react with?

This antibody reacts specifically with human Kappa light chains.

What is the recommended dilution and application for using this antibody in immunohistochemistry?

For immunohistochemistry on formalin-fixed, paraffin-embedded tissues, the concentrated antibody is recommended to be diluted between 1:100 and 1:200.

How should the mouse anti-Kappa monoclonal antibody (ZM81) be stored to maintain its stability?

For short-term storage, keep the antibody at 2-8°C, and for longer-term storage, it should be kept at -20°C while avoiding repeated freeze-thaw cycles.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.