Immunohistochemistry (IHC) : Human Hodgkin lymphoma stained with anti-Fascin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of Hodgkin’s cells.

mouse anti-Fascin monoclonal antibody (ZM192) 6182

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to Fascin
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2a
Control: Hodgkin’s lymphoma or spleen
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6182parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, Fascin, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Fascin monoclonal antibody ZM192 6182

antibody
Database link: human Q16658
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Hodgkin's lymphoma or spleen
Immunogen Full length recombinant human FSCN1 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2a - Isotype Control

target relevance
Homo sapiens FSCN1 Fascin
Protein names Fascin
Alternative names 55 kDa actin-bundling protein, Singed-like protein, p55
Gene names FSCN1
Protein family Belongs to the fascin family
Function Actin-binding protein that contains 2 major actin binding sites (PubMed:21685497, PubMed:23184945). Organizes filamentous actin into parallel bundles (PubMed:20393565, PubMed:21685497, PubMed:23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed:22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed:20393565, PubMed:21685497, PubMed:23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed:22155786)
Subcellular location Cytoplasm, cytosol, Cytoplasm, cell cortex, Cytoplasm, cytoskeleton, Cytoplasm, cytoskeleton, stress fiber, Cell projection, filopodium, Cell projection, invadopodium, Cell projection, microvillus, Cell junction
Structure Interacts with RUFY3 (via N-terminus); the interaction induces neuron axon development (By similarity). Interacts with NGFR (By similarity). Associates with CTNNB1 (By similarity). Interacts with PLXNB3 (PubMed:21706053)
Post-translational modification Phosphorylation at Ser-39 inhibits actin-binding (PubMed:8647875, PubMed:8999969). Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF (PubMed:22155786)
Keywords 3D-structure, Acetylation, Actin-binding, Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Direct protein sequencing, Isopeptide bond, Phosphoprotein, Proteomics identification, Reference proteome, Ubl conjugation
Sequence MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAV CLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLS CFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLA FQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTL KAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKN GQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFND GAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA SAETVDPASLWEY
UniProt accession: Q16658

Data

Human Hodgkin lymphoma stained with anti-Fascin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of Hodgkin's cells.

FAQ & Publications

Frequently Asked Questions

What species does the mouse anti-Fascin monoclonal antibody (ZM192) specifically react with?

This antibody specifically reacts with human Fascin protein.

For which application is the mouse anti-Fascin monoclonal antibody (ZM192) validated and recommended?

The antibody is validated and recommended for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues.

How should the mouse anti-Fascin monoclonal antibody (ZM192) be stored to maintain stability?

For short-term storage, keep the antibody at 2-8°C. For long-term storage, it should be kept at -20°C, and freeze/thaw cycles should be avoided.

What is the recommended dilution range when using the concentrated form of this antibody for IHC?

The recommended dilution for the concentrated antibody in IHC applications is between 1:100 and 1:200.

What is the immunogen used to generate the mouse anti-Fascin monoclonal antibody (ZM192)?

The antibody was raised against the full-length recombinant human FSCN1 protein.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.