Immunohistochemistry (IHC) : Human spleen stained with anti-CD22 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of B-lymphocytes.

mouse anti-CD22 monoclonal antibody (ZM183) 6077

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to CD22
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Lymph node
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6077parent Category: antibody Tags: anatomy, CD22, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-CD22 monoclonal antibody ZM183 6077

antibody
Database link: human P20273
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Lymph node
Immunogen Recombinant human BL-CAM/CD22 protein fragment (around aa 52-178)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names B-cell receptor CD22 (B-lymphocyte cell adhesion molecule) (BL-CAM) (Sialic acid-binding Ig-like lectin 2) (Siglec-2) (T-cell surface antigen Leu-14) (CD antigen CD22)
Gene names CD22,CD22 SIGLEC2
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass 95348Da
Function FUNCTION: Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed:34330755, PubMed:8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed:20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed:8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed:20516366). {ECO:0000269\|PubMed:20172905, ECO:0000269\|PubMed:20516366, ECO:0000269\|PubMed:34330755, ECO:0000269\|PubMed:8627166}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:20172905}; Single-pass type I membrane protein.
Tissues TISSUE SPECIFICITY: B-lymphocytes.
Structure SUBUNIT: Predominantly monomer of isoform CD22-beta. Also found as heterodimer of isoform CD22-beta and a shorter isoform. Interacts with PTPN6/SHP-1, LYN, SYK, PIK3R1/PIK3R2 and PLCG1 upon phosphorylation. Interacts with GRB2, INPP5D and SHC1 upon phosphorylation (By similarity). May form a complex with INPP5D/SHIP, GRB2 and SHC1. {ECO:0000250\|UniProtKB:P35329, ECO:0000269\|PubMed:7618087, ECO:0000269\|PubMed:8627166, ECO:0000269\|PubMed:8647200}.
Post-translational modification PTM: Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2. {ECO:0000250\|UniProtKB:P35329}.; PTM: Phosphorylated on tyrosine residues by LYN. {ECO:0000250\|UniProtKB:P35329}.
Domain DOMAIN: Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
Target Relevance information above includes information from UniProt accession: P20273
The UniProt Consortium

Data

Human spleen stained with anti-CD22 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of B-lymphocytes.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.