Immunohistochemistry (IHC) : Human tonsil stained with anti-CD21 antibodyusing peroxidase-conjugate and DAB chromogen. Note the membrane staining of follicular dendritic cells in follicles.

mouse anti-CD21 monoclonal antibody (ZM75) 6076

$160.00 – $528.00

Antibody summary

Mouse monoclonal to CD21
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Tonsil, lymph node
Visualization: Cell membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6076parent Category: antibody Tags: anatomy, CD21, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-CD21 monoclonal antibody ZM75 6076

antibody
Database link: human P20023
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil, lymph node
Immunogen Recombinant human CR2 (CD21) protein fragment (around aa 44-196)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Complement receptor type 2 (Cr2) (Complement C3d receptor) (Epstein-Barr virus receptor) (EBV receptor) (CD antigen CD21)
Gene names CR2,CR2 C3DR
Protein family Receptors of complement activation (RCA) family
Mass 112916Da
Function FUNCTION: Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1 (PubMed:1849076, PubMed:21527715, PubMed:7753047). When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells (PubMed:21527715). This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling (PubMed:1383329, PubMed:30107486). Also induces specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner (PubMed:12938232). Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production (PubMed:1386409). {ECO:0000269\|PubMed:12938232, ECO:0000269\|PubMed:1383329, ECO:0000269\|PubMed:1386409, ECO:0000269\|PubMed:1849076, ECO:0000269\|PubMed:21527715, ECO:0000269\|PubMed:30107486, ECO:0000269\|PubMed:7753047}.; FUNCTION: (Microbial infection) Acts as a receptor for Epstein-Barr virus. {ECO:0000269\|PubMed:2460635}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:1383329, ECO:0000269\|PubMed:1702139}; Single-pass type I membrane protein.
Tissues TISSUE SPECIFICITY: Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
Structure SUBUNIT: Interacts (via Sushi domain 1 and 2) with C3 (PubMed:11387479, PubMed:21527715). Interacts with CD19 (PubMed:1702139). Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells (PubMed:1383329). Interacts (via Sushi domain 1 and 2) with FCER2 (via the C-terminus). Interacts with CD23 (PubMed:1386409). Interacts with FCRL5 (PubMed:30107486). Interacts with CR1 (PubMed:1708808). Interacts with INFNA1 (PubMed:1849076). {ECO:0000269\|PubMed:11387479, ECO:0000269\|PubMed:1383329, ECO:0000269\|PubMed:1386409, ECO:0000269\|PubMed:16172256, ECO:0000269\|PubMed:1702139, ECO:0000269\|PubMed:1708808, ECO:0000269\|PubMed:1849076, ECO:0000269\|PubMed:21527715, ECO:0000269\|PubMed:30107486}.; SUBUNIT: (Microbial infection) Interacts with Epstein-Barr virus gp350 protein. {ECO:0000269\|PubMed:2460635}.
Involvement in disease DISEASE: Systemic lupus erythematosus 9 (SLEB9) [MIM:610927]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow. {ECO:0000269\|PubMed:17360460}. Note=Disease susceptibility is associated with variants affecting the gene represented in this entry.; DISEASE: Immunodeficiency, common variable, 7 (CVID7) [MIM:614699]: A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. {ECO:0000269\|PubMed:22035880}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P20023
The UniProt Consortium

Data

Human tonsil stained with anti-CD21 antibodyusing peroxidase-conjugate and DAB chromogen. Note the membrane staining of follicular dendritic cells in follicles.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.