Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD14 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining of Langerhans cells (in follicle) and histiocytes in the perifollicular region

mouse anti-CD14 monoclonal antibody (ZM104) 6419

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to CD14
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1/ Kappa
Control: Tonsil or lymph node
Visualization: Membrane and cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

Clear

SKU: 6419parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, CD14, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1/ Kappa
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-CD14 monoclonal antibody ZM104 6419

antibody
Database link: human P08571
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Tonsil or lymph node
Immunogen Recombinant fragment (around aa1-250) of human CD138 protein (exact sequence is proprietary)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Monocyte differentiation antigen CD14 (My23 antigen) (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form]
Gene names CD14,CD14
Mass 40076Da
Function FUNCTION: Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187). {ECO:0000269\|PubMed:16880211, ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:20133493, ECO:0000269\|PubMed:22265692, ECO:0000269\|PubMed:23264655, ECO:0000269\|PubMed:23880187, ECO:0000269\|PubMed:8612135}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:2462937, ECO:0000269\|PubMed:3385210}; Lipid-anchor, GPI-anchor {ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:2462937, ECO:0000269\|PubMed:3385210}. Secreted {ECO:0000269\|PubMed:25497142, ECO:0000269\|PubMed:2779588}. Membrane raft {ECO:0000269\|PubMed:16880211}. Golgi apparatus {ECO:0000269\|PubMed:16880211}. Note=Secreted forms may arise by cleavage of the GPI anchor. {ECO:0000269\|PubMed:2462937, ECO:0000269\|PubMed:2779588, ECO:0000269\|PubMed:3385210}.
Tissues TISSUE SPECIFICITY: Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. {ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:25497142}.
Structure SUBUNIT: Interacts with LPS-bound LPB (PubMed:1698311, PubMed:23264655). Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (PubMed:11274165). Interacts with LPAR1 (By similarity). Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively (PubMed:16880211). Interacts with MYO18A (PubMed:25965346). Interacts with FSTL1 (PubMed:22265692). {ECO:0000250\|UniProtKB:P10810, ECO:0000269\|PubMed:11274165, ECO:0000269\|PubMed:16880211, ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:22265692, ECO:0000269\|PubMed:23264655, ECO:0000269\|PubMed:25965346}.
Post-translational modification PTM: N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. {ECO:0000269\|PubMed:16335952, ECO:0000269\|PubMed:19159218, ECO:0000269\|PubMed:19838169}.
Domain DOMAIN: The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS. {ECO:0000250\|UniProtKB:P10810}.
Target Relevance information above includes information from UniProt accession: P08571
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human tonsil stained with anti-CD14 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining of Langerhans cells (in follicle) and histiocytes in the perifollicular region

FAQ & Publications

Frequently Asked Questions

What species does the mouse anti-CD14 monoclonal antibody (ZM104) react with?

This antibody is reactive with human CD14.

Which applications is the mouse anti-CD14 monoclonal antibody (ZM104) suitable for?

It is suitable for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues.

How should the mouse anti-CD14 monoclonal antibody (ZM104) be stored for optimal stability?

Store the antibody at 2-8°C for short-term use, and for longer-term storage keep at -20°C while avoiding repeated freeze/thaw cycles.

What is the recommended dilution range for using the concentrated mouse anti-CD14 monoclonal antibody (ZM104) in IHC?

The recommended dilution for the concentrated antibody is between 1:100 and 1:200 for immunohistochemistry applications.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.