Immunohistochemistry (IHC) : Human lymph node stained with anti-BOB1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of follicular center B cells .

mouse anti-BOB1 monoclonal antibody (ZM74) 6444

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to BOB1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2b
Control: Tonsil
Visualization: Cytoplasmic and nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6444parent Category: antibody Tags: anatomy, BOB1, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-BOB1 monoclonal antibody ZM74 6444

antibody
Database link: human Q16633
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Tonsil
Immunogen Recombinant full-length human beta-catenin (CTNNB1) protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2b - Isotype Control

target relevance
Protein names POU domain class 2-associating factor 1 (B-cell-specific coactivator OBF-1) (BOB-1) (OCA-B) (OCT-binding factor 1)
Gene names POU2AF1,POU2AF1 BOB1 OBF1
Protein family POU2AF family
Mass 27436Da
Function FUNCTION: Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity). {ECO:0000250\|UniProtKB:Q64693, ECO:0000269\|PubMed:7623806, ECO:0000269\|PubMed:7779176, ECO:0000269\|PubMed:7859290}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
Tissues TISSUE SPECIFICITY: B-cell specific (PubMed:7779176, PubMed:7859290). Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine (PubMed:7779176, PubMed:7859290). {ECO:0000269\|PubMed:7779176, ECO:0000269\|PubMed:7859290}.
Structure SUBUNIT: Interacts with POU2F1/OCT1 and POU2F2/OCT2; the interaction increases POU2F1 and POU2F2 transactivation activity. {ECO:0000269\|PubMed:7859290}.
Post-translational modification PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. {ECO:0000305\|PubMed:11483517}.
Domain DOMAIN: In the N-terminus possesses a conserved OCA domain for bivalent binding to class II POU domain-containing transcription factors and to an octamer DNA motif. {ECO:0000269\|PubMed:35576971}.
Involvement in disease DISEASE: Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. {ECO:0000269\|PubMed:8574789}.
Target Relevance information above includes information from UniProt accession: Q16633
The UniProt Consortium

Data

Human lymph node stained with anti-BOB1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of follicular center B cells .

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.