mouse anti-beta Tubulin monoclonal antibody (BT7R) 5007

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to beta Tubulin
Suitable for: WB,ICC/IF,ELISA
Reacts with: human, mouse, rat
Isotype: IgG2a
100 µg, 25 µg, 1 mg

SKU: 5007parent Categories: antibody, loading control Tag: beta Tubulin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-beta Tubulin monoclonal antibody (BT7R) 5007

antibody
Database link: human P07437 mouse P99024 rat P69897
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:2000-5000
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2a - Isotype Control

target relevance
Beta tubulin is a structural component of microtubules, beta-tubulin plays a fundamental role in maintaining cell shape, supporting intracellular transport, and facilitating cell division. Due to its stable expression levels and essential cellular functions, beta-tubulin has become widely recognized as a valuable loading control in various experimental techniques, such as Western blotting and immunoblotting. Its consistent presence in most eukaryotic cells ensures that its expression remains relatively constant, making it an ideal reference protein for normalizing and quantifying target protein levels accurately. This antibody can be used as a loading control when run alongside proteins of interest with different and resolvable molecular weights and ideally in combination with antibodies of same host and when using a secondary antibody. Click for more on: loading controls and beta Tubulin
Protein names Tubulin beta chain (Tubulin beta-5 chain)
Gene names TUBB,TUBB TUBB5 OK/SW-cl.56
Protein family Tubulin family
Mass 49671Da
Function Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Subellular location Cytoplasm, cytoskeleton
Tissues Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Structure Heterodimer of alpha and beta chains (PubMed:26637975). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with PIFO (PubMed:20643351). Interacts with DIAPH1 (PubMed:23325789). Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity). Nascent tubulin polypeptide interacts (via beta-tubulin MREI motif) with TTC5/STRAP; this interaction results in tubulin mRNA-targeted degradation (PubMed:31727855).
Post-translational modification Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866, PubMed:28576883). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity). ; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable) (PubMed:28576883). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable) (PubMed:28576883). ; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Involvement in disease Cortical dysplasia, complex, with other brain malformations 6 (CDCBM6) [MIM:615771]: A disorder of aberrant neuronal migration and disturbed axonal guidance. Affected individuals have microcephaly, ataxia, and severe delayed psychomotor development. Brain imaging shows variable malformations of cortical development, including white matter streaks, dysmorphic basal ganglia, corpus callosum abnormalities, brainstem and cerebellar hypoplasia, cortical dysplasia, polymicrogyria. Note=The disease is caused by variants affecting the gene represented in this entry.; Skin creases, congenital symmetric circumferential, 1 (CSCSC1) [MIM:156610]: An autosomal dominant disease characterized by multiple, symmetric, circumferential rings of folded skin, affecting primarily the limbs. Affected individuals also exhibit intellectual disability, cleft palate, and dysmorphic features. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P07437
The UniProt Consortium

Data

10µg/lane of mouse brain tissue lysates. 50kDa band is Anti-β-Tubulin (5007) at 1:2000 dilution (0.5µg/mL).

1:4000 (0.25µg/mL) Ab dilution used in WB of 10µg/lane (1), 5µg/lane (2), 2.5µg/lane (3), 1.25µg/lane (4) mouse brain tissue lysates.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.