Immunohistochemistry (IHC) : Human lymph node involved by hairy cell leukemia stained with anti-Annexin A1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear/cytoplasmic staining of tumor cells.

mouse anti-Annexin A1 monoclonal antibody (ZM211) 6023

$160.00 – $528.00

Antibody summary

Mouse monoclonal to Annexin A1
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2a
Control: Lymph node or hairy cell leukemia
Visualization: Nuclear, cytoplasmic, and membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6023parent Category: antibody Tags: anatomy, Annexin A1, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Annexin A1 monoclonal antibody ZM211 6023

antibody
Database link: human P04083
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Lymph node or hairy cell leukemia
Immunogen Recombinant full-length human Annexin A1 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2a - Isotype Control

target relevance
Protein names Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35) [Cleaved into: Annexin Ac2-26]
Gene names ANXA1,ANXA1 ANX1 LPC1
Protein family Annexin family
Mass 38714Da
Function FUNCTION: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). {ECO:0000250\|UniProtKB:P10107, ECO:0000250\|UniProtKB:P19619, ECO:0000269\|PubMed:17008549, ECO:0000269\|PubMed:19625660, ECO:0000269\|PubMed:2532504, ECO:0000269\|PubMed:2936963, ECO:0000269\|PubMed:8425544, ECO:0000269\|PubMed:8557678}.; FUNCTION: [Annexin Ac2-26]: Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:15187149, PubMed:22879591, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591). {ECO:0000269\|PubMed:15187149, ECO:0000269\|PubMed:22879591, ECO:0000269\|PubMed:25664854}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269\|PubMed:10772777, ECO:0000269\|PubMed:19625660}. Cytoplasm {ECO:0000269\|PubMed:10772777, ECO:0000269\|PubMed:17008549, ECO:0000269\|PubMed:19625660}. Cell projection, cilium {ECO:0000250\|UniProtKB:P46193}. Cell membrane {ECO:0000269\|PubMed:10772777}. Membrane {ECO:0000269\|PubMed:17008549, ECO:0000269\|PubMed:2532504, ECO:0000269\|PubMed:8557678}; Peripheral membrane protein {ECO:0000269\|PubMed:2532504, ECO:0000269\|PubMed:8557678}. Endosome membrane {ECO:0000250\|UniProtKB:P07150}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P07150}. Basolateral cell membrane {ECO:0000250\|UniProtKB:P51662}. Apical cell membrane {ECO:0000250\|UniProtKB:P10107}. Lateral cell membrane {ECO:0000250\|UniProtKB:P10107}. Secreted {ECO:0000269\|PubMed:17008549, ECO:0000269\|PubMed:19625660, ECO:0000269\|PubMed:25664854}. Secreted, extracellular space {ECO:0000269\|PubMed:25664854}. Cell membrane {ECO:0000269\|PubMed:10772777, ECO:0000269\|PubMed:19625660, ECO:0000269\|PubMed:25664854}; Peripheral membrane protein {ECO:0000269\|PubMed:10772777, ECO:0000269\|PubMed:19625660, ECO:0000269\|PubMed:25664854}; Extracellular side {ECO:0000269\|PubMed:10772777, ECO:0000269\|PubMed:19625660, ECO:0000269\|PubMed:25664854}. Secreted, extracellular exosome {ECO:0000269\|PubMed:25664854}. Cytoplasmic vesicle, secretory vesicle lumen {ECO:0000269\|PubMed:10772777}. Cell projection, phagocytic cup {ECO:0000250\|UniProtKB:P10107}. Early endosome {ECO:0000250\|UniProtKB:P19619}. Cytoplasmic vesicle membrane {ECO:0000250\|UniProtKB:P19619}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P19619}. Note=Secreted, at least in part via exosomes and other secretory vesicles. Detected in exosomes and other extracellular vesicles (PubMed:25664854). Alternatively, the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in the protein translocation from the cytoplasm into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). Detected in gelatinase granules in resting neutrophils (PubMed:10772777). Secretion is increased in response to wounding and inflammation (PubMed:25664854). Secretion is increased upon T-cell activation (PubMed:17008549). Neutrophil adhesion to endothelial cells stimulates secretion via gelatinase granules, but foreign particle phagocytosis has no effect (PubMed:10772777). Colocalizes with actin fibers at phagocytic cups (By similarity). Displays calcium-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). {ECO:0000250\|UniProtKB:P10107, ECO:0000269\|PubMed:10772777, ECO:0000269\|PubMed:17008549, ECO:0000269\|PubMed:2532504, ECO:0000269\|PubMed:25664854, ECO:0000269\|PubMed:32272059, ECO:0000269\|PubMed:8557678}.
Tissues TISSUE SPECIFICITY: Detected in resting neutrophils (PubMed:10772777). Detected in peripheral blood T-cells (PubMed:17008549). Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors (PubMed:25664854). Detected in placenta (at protein level) (PubMed:2532504). Detected in liver. {ECO:0000269\|PubMed:2532504, ECO:0000269\|PubMed:2936963}.
Structure SUBUNIT: Homodimer; non-covalently linked (By similarity). Homodimer; linked by transglutamylation (PubMed:2532504). Homodimers linked by transglutamylation are observed in placenta, but not in other tissues (PubMed:2532504). Interacts with S100A11 (PubMed:10673436, PubMed:8557678). Heterotetramer, formed by two molecules each of S100A11 and ANXA1 (PubMed:10673436). Interacts with DYSF (By similarity). Interacts with EGFR (By similarity). {ECO:0000250\|UniProtKB:P10107, ECO:0000250\|UniProtKB:P19619, ECO:0000269\|PubMed:10673436, ECO:0000269\|PubMed:2532504, ECO:0000269\|PubMed:8557678}.
Post-translational modification PTM: Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:15485879, PubMed:2457390). Phosphorylated in response to EGF treatment (PubMed:2532504). {ECO:0000269\|PubMed:15485879, ECO:0000269\|PubMed:2457390, ECO:0000269\|PubMed:2532504}.; PTM: Sumoylated. {ECO:0000250\|UniProtKB:P10107}.; PTM: Proteolytically cleaved by cathepsin CTSG to release the active N-terminal peptide Ac2-26. {ECO:0000269\|PubMed:22879591}.
Domain DOMAIN: The full-length protein can bind eight Ca(2+) ions via the annexin repeats. Calcium binding causes a major conformation change that modifies dimer contacts and leads to surface exposure of the N-terminal phosphorylation sites; in the absence of Ca(2+), these sites are buried in the interior of the protein core. The N-terminal region becomes disordered in response to calcium-binding. {ECO:0000250\|UniProtKB:P19619}.
Target Relevance information above includes information from UniProt accession: P04083
The UniProt Consortium

Data

Human lymph node involved by hairy cell leukemia stained with anti-Annexin A1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear/cytoplasmic staining of tumor cells.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.