| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| target | Legionella |
| species reactivity | For the detection of Legionella pneumophila |
| applications | RT PCR |
| assay type | direct & qualitative |
| available sizes | 96 tests |
Legionella RT-PCR test Mikrogen 830539
$487.00
Summary
- Mikrogen diagnostik RT PCR kit for research use (RUO)
- Direct Legionella pneumophila detection
- High sensitivity and specificity
- Internal control for monitoring nucleic acid extraction
(RNA/DNA) and real-time PCR inhibition in each reaction - Compatible with most common real-time PCR cyclers & RNA/DNA extraction methods
- 96 tests
Legionella RT-PCR test Mikrogen 830539
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| Assay type RT PCR | |||||||||||||||
| Research area Infectious Disease | |||||||||||||||
| Sample type whole blood, serum, plasma, urine, tissue, stool, etc., food and environmental samples or from the carrier material | |||||||||||||||
Notes
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Components
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| Storage Store at -20°C. | |||||||||||||||
| Additional information Highly sensitive and specific direct detection of pathogens that can cause tick-borne infections
Applicable to human starting material as well as RNA/DNA from the tick |
| target relevance |
|---|
| Organism Legionella pneumophila |
| Structure and strains Legionella pneumophila is an aerobic, pleomorphic, flagellated, non-spore-forming, Gram-negative bacterium of the genus Legionella. L. pneumophila is the primary human pathogenic bacterium in this group. In nature, L. pneumophila infects freshwater and soil amoebae of the genera Acanthamoeba and Naegleria. This pathogen is found commonly near freshwater environments and will then invade the amoebae found in these environments, using them to carry out metabolic functions. L. pneumophila is also the causative agent of Legionnaires' disease, also known as legionellosis. Due to L. pneumophila s ability to thrive in water, it can grow in water filtration systems, leading to faucets, showers, and other fixtures and then spread through aerosolized water droplets. Once infected, this bacterium can cause pneumonia with symptoms such as fever, delirium, diarrhea, and decreased liver and kidney function. After its initial discovery, it was found that Legionella replicates inside amoeba, which suggests that bacteria replicating in environmental protozoa have the ability to exploit signaling pathways in human phagocytic cells. |
| Disease The family of legionellaceae comprises more than 39 different species, which are subdivided into various serovars. The most important human is Legionella pneumophila which comprises 14 serovars. About 80 to 85% of all Legionella associated pneumonias are caused by serovars 1 to 6. The typical legionellosis (Legionnaires disease) starts two to ten days after infection with uncharacteristic symptoms such as general indisposition, headache, muscle aches and dry cough, chest pain, chill, temperature rise to 39 to over 40 °C. Occasionally, gastrointestinal disturbances accompanied by diarrhoea and vomiting may be early onset symptoms. In 2 to 5% of cases, severe pneumonia may result from the infection by Legionella pneumophila with lethality varying from 10 to 20%. If not treated, lethality of immunosuppressed patients may increase up to 80%. The so called pontiac fever is a benign, non-pulmonary form of legionellosis characterized by a short incubation period of one to two days and mild, flu-like course of disease. The disease starts with headache, muscle- and chest pain, cough and fever. Despite severe discomfort, patients generally recover completely within five days. |
| Detection and diagnosis Due to the non-specific nature of the symptoms, diagnosis is primarily based upon the results of laboratory analyses. Rapid tests, which are also suitable for screening purposes, demonstrate the presence of Legionella specific antigens in patient's urine while serological methods serve to establish a differential diagnosis in cases of atypical pneumonia. The SERION ELISA classic Legionella pneumophila 1-7 IgG and IgM tests are based on a combination of diagnostically relevant antigens from the serovars 1 to 7 |
Data
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Publications
Publications
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| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
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| 830539 protocol |
Documents
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