Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded succinate dehydrogenase deficient RCC stained with anti-SDHB antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

rabbit anti-SDHB monoclonal antibody (ZR339) 6362

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to SDHB
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Paraganglioma
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6362parent Category: antibody Tags: anatomy, SDHB, Urinary Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-SDHB monoclonal antibody ZR339 6362

antibody
Database link: human P21912
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Paraganglioma
Immunogen Synthetic peptide corresponding to residues in human SDHB was used as an immunogen
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC 1.3.5.1) (Iron-sulfur subunit of complex II) (Ip) (Malate dehydrogenase [quinone] iron-sulfur subunit) (EC 1.1.5.-)
Protein family Succinate dehydrogenase/fumarate reductase iron-sulfur protein family
Mass 31630Da
Function Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:26925370, PubMed:27604842). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity).
Catalytic activity CATALYTIC ACTIVITY: Reaction=a quinone + succinate = a quinol + fumarate; Xref=Rhea:RHEA:40523, ChEBI:CHEBI:24646, ChEBI:CHEBI:29806, ChEBI:CHEBI:30031, ChEBI:CHEBI:132124; EC=1.3.5.1; Evidence=; CATALYTIC ACTIVITY: Reaction=(R)-malate + a quinone = a quinol + enol-oxaloacetate; Xref=Rhea:RHEA:79827, ChEBI:CHEBI:15588, ChEBI:CHEBI:17479, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:79828; Evidence=; CATALYTIC ACTIVITY: Reaction=(S)-malate + a quinone = a quinol + enol-oxaloacetate; Xref=Rhea:RHEA:79831, ChEBI:CHEBI:15589, ChEBI:CHEBI:17479, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:79832; Evidence=;
Pathway PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Subellular location Mitochondrion inner membrane ; Peripheral membrane protein ; Matrix side .
Structure Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD (PubMed:37098072). Interacts with SDHAF1; the interaction is required for iron-sulfur cluster incorporation into SDHB (PubMed:26749241).; (Microbial infection) Interacts with JC virus small t antigen.
Domain DOMAIN 40..133; /note="2Fe-2S ferredoxin-type"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU00465"; DO
Involvement in disease Pheochromocytoma/paraganglioma syndrome 4 (PPGL4) [MIM:115310]: A form of pheochromocytoma/paraganglioma syndrome, a tumor predisposition syndrome characterized by the development of neuroendocrine tumors, usually in adulthood. Pheochromocytomas are catecholamine-producing tumors that arise from chromaffin cells in the adrenal medulla. Paragangliomas develop from sympathetic paraganglia in the thorax, abdomen, and pelvis, as well as from parasympathetic paraganglia in the head and neck. PPGL4 inheritance is autosomal dominant. Note=The disease is caused by variants affecting the gene represented in this entry.; Paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]: Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Note=The disease is caused by variants affecting the gene represented in this entry.; Mitochondrial complex II deficiency, nuclear type 4 (MC2DN4) [MIM:619224]: A form of mitochondrial complex II deficiency, a disorder with heterogeneous clinical manifestations. Some patients have multisystem involvement of the brain, heart, muscle, liver, and kidneys resulting in death in infancy, whereas others have only isolated cardiac or muscle involvement with onset in adulthood and normal cognition. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome. MC2DN4 is a severe, autosomal recessive form characterized by early-onset progressive neurodegeneration with leukoencephalopathy. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P21912
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded succinate dehydrogenase deficient RCC stained with anti-SDHB antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.