Immunohistochemistry (IHC) : Human tonsil stained with anti-Lambda light chain antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of plasma cells.

mouse anti-Lambda monoclonal antibody (LcN-2) 6239

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to Lambda
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2a
Control: Tonsil
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6239parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, Hematopathology, Lambda

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Lambda monoclonal antibody LcN-2 6239

antibody
Database link: human P01703
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Tonsil
Immunogen Purified human IgG
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2a - Isotype Control

target relevance
Protein names Immunoglobulin lambda variable 1-40 (Ig lambda chain V-I region NEWM)
Gene names IGLV1-40,IGLV1-40
Mass 12302Da
Function FUNCTION: V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). {ECO:0000303\|PubMed:17576170, ECO:0000303\|PubMed:20176268, ECO:0000303\|PubMed:22158414, ECO:0000303\|PubMed:24600447}.
Subellular location SUBCELLULAR LOCATION: Secreted {ECO:0000303\|PubMed:20176268, ECO:0000303\|PubMed:22158414}. Cell membrane {ECO:0000303\|PubMed:20176268, ECO:0000303\|PubMed:22158414}.
Structure SUBUNIT: Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked. {ECO:0000303\|PubMed:20176268}.
Target Relevance information above includes information from UniProt accession: P01703
The UniProt Consortium

Data

Human tonsil stained with anti-Lambda light chain antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of plasma cells.

FAQ & Publications

Frequently Asked Questions

What is the recommended application and dilution for the mouse anti-Lambda monoclonal antibody (LcN-2) 6239?

This antibody is suitable for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded human tissues. The recommended dilution for the concentrated antibody is 1:100 to 1:200.

How should the mouse anti-Lambda monoclonal antibody LcN-2 (6239) be stored to maintain stability?

For short-term storage, keep the antibody at 2-8°C. For long-term storage, it should be kept at -20°C. It is important to avoid repeated freeze/thaw cycles to preserve antibody integrity.

Which secondary antibodies are compatible with the mouse anti-Lambda monoclonal antibody (LcN-2) 6239 for detection?

Compatible secondary antibodies include goat anti-mouse IgG, H&L chain specific polyclonal antibodies conjugated to peroxidase, biotin, or FITC. Both crossabsorbed and non-crossabsorbed variants are suitable, such as antibodies with catalog numbers 5486, 2685, 7854, 1706, 1716, and 1721.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.