Immunohistochemistry (IHC) : Human Hodgkin lymphoma stained with anti-Fascin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of Hodgkin’s cells.

mouse anti-Fascin monoclonal antibody (ZM192) 6182

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to Fascin
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2a
Control: Hodgkin’s lymphoma or spleen
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6182parent Category: antibody Tags: anatomy, Fascin, Hematopathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Fascin monoclonal antibody ZM192 6182

antibody
Database link: human Q16658
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Hodgkin's lymphoma or spleen
Immunogen Full length recombinant human FSCN1 protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2a - Isotype Control

target relevance
Protein names Fascin (55 kDa actin-bundling protein) (Singed-like protein) (p55)
Gene names FSCN1,FSCN1 FAN1 HSN SNL
Protein family Fascin family
Mass 54530Da
Function FUNCTION: Actin-binding protein that contains 2 major actin binding sites (PubMed:21685497, PubMed:23184945). Organizes filamentous actin into parallel bundles (PubMed:20393565, PubMed:21685497, PubMed:23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed:22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed:20393565, PubMed:21685497, PubMed:23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed:22155786). {ECO:0000269\|PubMed:20137952, ECO:0000269\|PubMed:20393565, ECO:0000269\|PubMed:21685497, ECO:0000269\|PubMed:22155786, ECO:0000269\|PubMed:23184945, ECO:0000269\|PubMed:9362073, ECO:0000269\|PubMed:9571235}.
Subellular location SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269\|PubMed:21706053, ECO:0000269\|PubMed:9571235}. Cytoplasm, cell cortex {ECO:0000269\|PubMed:21685497}. Cytoplasm, cytoskeleton {ECO:0000269\|PubMed:21685497, ECO:0000269\|PubMed:3525578, ECO:0000269\|PubMed:9571235}. Cytoplasm, cytoskeleton, stress fiber {ECO:0000269\|PubMed:21706053}. Cell projection, filopodium {ECO:0000269\|PubMed:20137952, ECO:0000269\|PubMed:21685497, ECO:0000269\|PubMed:3525578}. Cell projection, invadopodium {ECO:0000269\|PubMed:20137952}. Cell projection, microvillus {ECO:0000269\|PubMed:9571235}. Cell junction {ECO:0000269\|PubMed:9571235}. Note=Colocalized with RUFY3 and F-actin at filipodia of the axonal growth cone. Colocalized with DBN1 and F-actin at the transitional domain of the axonal growth cone (By similarity). {ECO:0000250\|UniProtKB:Q61553, ECO:0000269\|PubMed:21706053}.
Tissues TISSUE SPECIFICITY: Ubiquitous.
Structure SUBUNIT: Interacts with RUFY3 (via N-terminus); the interaction induces neuron axon development (By similarity). Interacts with NGFR (By similarity). Associates with CTNNB1 (By similarity). Interacts with PLXNB3 (PubMed:21706053). {ECO:0000250\|UniProtKB:P85845, ECO:0000250\|UniProtKB:Q61553, ECO:0000269\|PubMed:21706053}.
Post-translational modification PTM: Phosphorylation at Ser-39 inhibits actin-binding (PubMed:8647875, PubMed:8999969). Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF (PubMed:22155786). {ECO:0000269\|PubMed:22155786, ECO:0000269\|PubMed:8647875, ECO:0000269\|PubMed:8999969}.
Domain DOMAIN: Composed of four fascin beta-trefoil domains. {ECO:0000269\|PubMed:20393565, ECO:0000269\|PubMed:20434460, ECO:0000269\|PubMed:21685497, ECO:0000269\|PubMed:23184945}.
Target Relevance information above includes information from UniProt accession: Q16658
The UniProt Consortium

Data

Human Hodgkin lymphoma stained with anti-Fascin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of Hodgkin's cells.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.