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rabbit anti-Vimentin polyclonal antibody 5866


Antibody summary

  • Rabbit polyclonal to Vimentin
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat
  • Isotype: IgG
  • 100 µL, 25 µL, 1 mL
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SKU: 5866parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in










available sizes

1 mL, 100 µL, 25 µL

Database link:
human P08670
mouse P20152
rat P31000
Tested applications
Recommended dilutions
Western blot: 1:10000 IF/ICC and IHC: 1:5000
Full length human TH expressed in and purified from E. coli.
Size and concentration
25, 100, 1000µL and serum
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
serum, 0.04% NaN3 added
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Vimentin, a key protein constituent of the intermediate filament cytoskeleton, plays a pivotal role in maintaining cellular integrity and structure. In research, vimentin has become a valuable biomarker due to its tissue-specific expression and involvement in various cellular processes. Immunohistochemical analysis of vimentin has been extensively utilized to identify and characterize mesenchymal cells, such as fibroblasts, endothelial cells, and immune cells, aiding in the study of tissue development, wound healing, and cancer progression. Additionally, vimentin has been linked to epithelial-mesenchymal transition (EMT), a critical process implicated in embryonic development and metastasis. Furthermore, efforts to develop vimentin-targeting therapies for cancer treatment have shown promise, as vimentin's involvement in EMT and tumor progression make it a potential therapeutic target for limiting cancer metastasis.

Click for more on: cell markers and Vimentin
Protein names
Gene names
Protein family
Intermediate filament family
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. ; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Subellular location
Cytoplasm Cytoplasm, cytoskeleton Nucleus matrix Cell membrane
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Homomer assembled from elementary dimers (PubMed:20176112). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Interacts with BCAS3 (PubMed:17505058). Interacts with LGSN (By similarity). Interacts with SYNM (By similarity). Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with PLEC isoform 1C (PubMed:24940650). Interacts with STK33 (PubMed:18811945). Interacts with LARP6 (PubMed:21746880). Interacts with RAB8B (By similarity). Interacts with TOR1A; the interaction associates TOR1A with the cytoskeleton (PubMed:16361107, PubMed:18827015). Interacts with TOR1AIP1 (PubMed:16361107). Interacts with DIAPH1 (PubMed:23325789). Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament (PubMed:16923132). Interacts with the non-receptor tyrosine kinase SRMS; the interaction leads to phosphorylation of VIM (PubMed:29496907). Interacts with NOD2 (PubMed:27812135). Interacts (via head region) with CORO1C (By similarity). Interacts with HDGF (isoform 2) (PubMed:26845719). Interacts with PRKCE (via phorbol-ester/DAG-type 2 domain) (PubMed:18408015). Interacts with BFSP2 (By similarity). Interacts with PPL (By similarity). Interacts (via rod domain) with PKP1 (PubMed:10852826). Interacts with PKP2 (PubMed:10852826). ; (Microbial infection) Interacts with HCV core protein.
Post-translational modification
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480). Phosphorylated by STK33 (PubMed:18811945). Phosphorylated on tyrosine residues by SRMS (PubMed:29496907). ; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. ; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Target Relevance information above includes information from UniProt accession : P08670
The UniProt Consortium


Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, 5866, dilution 1:5,000, in green, and mouse mAb to Actin, 7047, dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Chromogenic immunostaining of a 4% PFA fixed paraffin embedded mouse kidney section with rabbit pAb to vimentin, 5866, dilution 1:1,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with Borg buffer retrieval (Biocare Medical). Hematoxylin (blue) was used as the counterstain. In this image, the 5866 antibody specifically labels the interstitium in glomerular podocytes. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues with high pH, EDTA based retrieval. Mouse select image for larger view.
Western blot analysis of whole cell lysates using rabbit pAb to vimentin, 5866, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa.


Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

35129203Fibroblast activation protein-alpha knockdown suppresses prostate cancer cell invasion and proliferationAn J, Hou D, Wang L, Wang L, Yang Y, Wang H.Histol Histopathol. 2022 Jun;37(6):597-607. doi: 10.14670/HH-18-430. Epub 2022 Feb 7.
33245857Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular MorphologiesCortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R.Cell Host Microbe. 2020 Dec 9;28(6):853-866.e5. doi: 10.1016/j.chom.2020.11.003. Epub 2020 Nov 17.
31248980Platelet-Specific Deletion of Cyclooxygenase-1 Ameliorates Dextran Sulfate Sodium-Induced Colitis in MiceSacco A, Bruno A, Contursi A, Dovizio M, Tacconelli S, Ricciotti E, Guillem-Llobat P, Salvatore T, Di Francesco L, Fullone R, Ballerini P, Arena V, Alberti S, Liu G, Gong Y, Sgambato A, Patrono C, FitzGerald GA, Yu Y, Patrignani P.J Pharmacol Exp Ther. 2019 Sep;370(3):416-426. doi: 10.1124/jpet.119.259382. Epub 2019 Jun 27.
30015869miR-577 suppresses cell proliferation and epithelial-mesenchymal transition by regulating the WNT2B mediated Wnt/β-catenin pathway in non-small cell lung cancerWang B, Sun L, Li J, Jiang R.Mol Med Rep. 2018 Sep;18(3):2753-2761. doi: 10.3892/mmr.2018.9279. Epub 2018 Jul 16.
28653599[ARTICLE WITHDRAWN] MicroRNA-539 Inhibits the Epithelial-Mesenchymal Transition of Esophageal Cancer Cells by Twist-Related Protein 1-Mediated Modulation of Melanoma-Associated Antigen A4Cao Z, Zheng X, Cao L, Liang N.Oncol Res. 2018 May 7;26(4):529-536. doi: 10.3727/096504017X14972679378357. Epub 2017 Jun 12.
28450710Targeted Exome Sequencing of Congenital Cataracts Related Genes: Broadening the Mutation Spectrum and Genotype-Phenotype Correlations in 27 Chinese Han FamiliesZhai Y, Li J, Yu W, Zhu S, Yu Y, Wu M, Sun G, Gong X, Yao K.Sci Rep. 2017 Apr 27;7(1):1219. doi: 10.1038/s41598-017-01182-9.
25187410Vimentin-a potential biomarker for therapeutic efficiency of HAARTJia X, Liu L, Liu X, Wu D, Yin L, Liu X, Zhang J, Yang P, Lu H, Zhang L.Acta Biochim Biophys Sin (Shanghai). 2014 Nov;46(11):1001-6. doi: 10.1093/abbs/gmu075. Epub 2014 Sep 3.
22903723Discovery of lamin B1 and vimentin as circulating biomarkers for early hepatocellular carcinomaWong KF, Luk JM.Methods Mol Biol. 2012;909:295-310. doi: 10.1007/978-1-61779-959-4_19.
21637948Vimentin in cancer and its potential as a molecular target for cancer therapySatelli A, Li S.Cell Mol Life Sci. 2011 Sep;68(18):3033-46. doi: 10.1007/s00018-011-0735-1. Epub 2011 Jun 3.
19126778Dominant cataract formation in association with a vimentin assembly disrupting mutationMüller M, Bhattacharya SS, Moore T, Prescott Q, Wedig T, Herrmann H, Magin TM.Hum Mol Genet. 2009 Mar 15;18(6):1052-7. doi: 10.1093/hmg/ddn440. Epub 2009 Jan 6.
7285936Vimentin, the 57 000 molecular weight protein of fibroblast filaments, is the major cytoskeletal component in immature gliaDahl D, Rueger DC, Bignami A, Weber K, Osborn M.Eur J Cell Biol. 1981 Jun;24(2):191-6.
6799297An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brainShaw G, Osborn M, Weber K.Eur J Cell Biol. 1981 Dec;26(1):68-82.


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