Description Determined by a competitive inhibition ELISA with nucleoside variants. The %B/B0 is plotted as a function of the concentration (nM) of the indicated competitor. 100% B/B0 indicates no inhibition by the indicated competitor.

Description Selectivity and Specificity of 6E4 in DNA: Binds 8-oxoA in single and double stranded DNA Recognizes 8-oxoA in all DNA base mispairs

Description Selectivity and Specificity of 6E4 in RNA: Binds 8-oxoA in single and double stranded RNA Recognizes 8-oxoA in RNA base mispairs with standard geometry

mouse anti-8-oxo Adenosine DNA/RNA damage monoclonal antibody (6E4) 1002

Price range: $320.00 through $450.00

Antibody summary

Mouse monoclonal to 8-oxo-adenosine
Suitable for: ELISA
Reacts with: All
Isotype: IgG1
100 µg, 500 µg

available sizes

Clear

SKU: 1002parent Categories: antibody, oxidative stress Tag: DNA/RNA damage

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA
reactivity	all, human
available sizes	100 µg, 500 µg

mouse anti-8-oxo Adenosine DNA/RNA damage monoclonal antibody (6E4) 1002

antibody

Tested applications ELISA
Recommended dilutions Dilute in PBS or medium that is identical to that used in the assay system. Optimal concentrations must be derived for specific applications.
Immunogen 8-oxo-adenosine
Size and concentration 100, 500µg and 1 mg/mL
Form liquid
Storage Instructions Store antibody at -20C to -80C. Avoid multiple freeze-thaw cycles.
Storage buffer PBS, pH 7.4
Purity protein G affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance

8-oxo-adenosine

adenosine

Alternative names
Adenine riboside, 9-beta-D-ribofuranosyladenine

Function
Adenosine participates in purine metabolism and extracellular signaling. It can act through adenosine receptors to regulate inflammation, vascular tone, cardiac conduction, neurotransmission, and immune responses.

Subcellular location
Adenosine is present intracellularly as part of purine metabolism and can also accumulate extracellularly, especially during hypoxia, inflammation, tissue stress, or ATP breakdown.

Description
Adenosine is an endogenous purine nucleoside composed of adenine linked to ribose. It functions as a signaling molecule, metabolic intermediate, and precursor of nucleotides such as AMP, ADP, ATP, cAMP, NAD, and RNA.

Chemical description
Purine nucleoside
C10H13N5O4
267.24 Da
0: Adenine; 1: Ribose

Biological role
Purine nucleoside
Metabolic intermediate
Extracellular signaling molecule
Precursor of AMP, ADP and ATP

Pathways
Purine metabolism
ATP metabolism
Adenosine receptor signaling
cAMP-mediated signaling

Diagnostic relevance
Adenosine and adenosine-pathway markers may be relevant in studies of inflammation, immune regulation, hypoxia, cardiovascular biology, cancer biology, and purine metabolism.

Research relevance
Adenosine is commonly studied as an immunomodulatory and cardioregulatory molecule. In immunology and oncology, extracellular adenosine is often associated with suppression of immune cell activation through adenosine receptor signaling.

Keywords
Nucleoside, Purine, Purine metabolism, Immune regulation, Adenosine receptor ligand

Structure
Adenosine consists of adenine attached to a ribose sugar through a beta-N9-glycosidic bond.

Function
Adenosine acts as both a metabolic intermediate and an extracellular signaling molecule. It is generated from ATP breakdown and can influence immune, cardiovascular, neurological, and inflammatory responses.

Pathway
Adenosine is part of purine metabolism and nucleotide turnover. It can be phosphorylated to AMP or deaminated to inosine.

Research relevance
Adenosine is relevant to assays studying inflammation, immune suppression, hypoxia, cancer microenvironments, cardiac signaling, and purine metabolism.

Data

Determined by a competitive inhibition ELISA with nucleoside variants. The %B/B0 is plotted as a function of the concentration (nM) of the indicated competitor. 100% B/B0 indicates no inhibition by the indicated competitor

Selectivity and Specificity of 6E4 in RNA: Binds 8-oxoA in single and double stranded RNA Recognizes 8-oxoA in RNA base mis-pairs with standard geometry

Selectivity and Specificity of 6E4 in DNA: Binds 8-oxoA in single and double stranded DNA Recognizes 8-oxoA in all DNA base mispairs

FAQ & Publications

Frequently Asked Questions

What applications is the mouse anti-8-oxo Adenosine monoclonal antibody (6E4) validated for?

This antibody is tested and validated for ELISA applications.

What are the recommended storage conditions for maintaining the stability of this antibody?

Store the antibody at -20°C to -80°C and avoid multiple freeze-thaw cycles to preserve its integrity.

Which species does the mouse anti-8-oxo Adenosine antibody react with?

The antibody is reactive with all species, including human samples.

Publications

pmid	title	authors	citation
36989571	Discovery and properties of a monoclonal antibody targeting 8-oxoA, an oxidized adenine lesion in DNA and RNA.	Daniel J Tew, Jakob M Hebert, Brad J Schmier	Redox Biol 62:102658

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.