mouse anti-8-hydroxy Guanosine DNA/RNA damage monoclonal antibody (15A3) 1102

Price range: $350.00 through $900.00

Antibody summary

Mouse monoclonal to 8-hydroxy-guanosine
Suitable for: ELISA, IHC, ICC, WB
Reacts with: All
Isotype: IgG2b
100 µg, 500 µg

available sizes

Clear

SKU: 1102parent Categories: antibody, oxidative stress Tags: 8-hydroxy-guanosine, DNA/RNA damage

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC, IHC, WB
reactivity	all, human
available sizes	100 µg, 500 µg

mouse anti-8-hydroxy Guanosine DNA/RNA damage monoclonal antibody (15A3) 1102

antibody

Tested applications ELISA, ICC, IHC, WB
Recommended dilutions Dilute in PBS or medium that is identical to that used in the assay system. Optimal concentrations must be derived for specific applications.
Immunogen 8-hydroxy-guanosine
Size and concentration 100, 500µg and 1 mg/mL
Form liquid
Storage Instructions Store antibody at -20C to -80C. Avoid multiple freeze-thaw cycles.
Storage buffer PBS, pH 7.4
Purity protein G affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2b - Isotype Control

target relevance

8-hydroxy-guanosine

guanosine

Alternative names
Guanine riboside, 9-beta-D-ribofuranosylguanine

Function
Guanosine serves as a precursor for GMP, GDP, and GTP. Through these nucleotides it contributes to RNA synthesis, signal transduction, protein translation, and numerous enzymatic reactions. Extracellular guanosine has also been implicated in neuroprotective and neuromodulatory activities.

Subcellular location
Guanosine is present intracellularly as part of nucleotide metabolism and may also be found extracellularly following nucleotide degradation or cellular release.

Description
Guanosine is a naturally occurring purine nucleoside consisting of guanine linked to a ribose sugar. It serves as a precursor for guanine nucleotides and participates in nucleic acid metabolism, cellular signaling, and energy transfer pathways.

Chemical description
Purine nucleoside
C10H13N5O5
283.24 Da
0: Guanine; 1: Ribose

Biological role
Purine nucleoside
Precursor of GMP
Precursor of GDP and GTP
RNA biosynthesis intermediate
Cellular signaling precursor

Pathways
Purine metabolism
Guanine nucleotide biosynthesis
RNA metabolism
GTP-dependent signaling

Diagnostic relevance
Alterations in guanosine and guanine nucleotide metabolism have been investigated in neurological disorders, cancer, metabolic diseases, and conditions involving altered purine metabolism.

Research relevance
Guanosine is widely studied in purine metabolism, nucleotide signaling, neurobiology, RNA biology, and cellular energy regulation. Guanosine-derived nucleotides such as GTP are critical regulators of G-proteins, microtubule dynamics, and protein synthesis.

Keywords
Nucleoside, Purine, Purine metabolism, RNA synthesis, GTP precursor

Structure
Guanosine consists of the purine base guanine attached to a ribose sugar through a beta-N9-glycosidic bond.

Function
Guanosine is a precursor of guanine nucleotides including GMP, GDP, and GTP. These molecules participate in RNA synthesis, signal transduction, protein translation, and cellular regulation.

Pathway
Guanosine is a central intermediate in purine metabolism and can be phosphorylated to GMP or generated through nucleotide degradation pathways.

Research relevance
Guanosine and its derivatives are important in studies of nucleotide metabolism, G-protein signaling, RNA biology, neuroprotection, and cancer metabolism.

Data

Distribution of 8-oxoG in (A) H2O2-treated Chlamydomonas and (B) Arsenite-treated HeLIamge generated William Zearges, Concordia University.

Distribution of 8-oxoG in (A) H2O2-treated Chlamydomonas and (B) Arsenite-treated HeLa. William Zearges, Concordia University.

FAQ & Publications

Frequently Asked Questions

What applications is the mouse anti-8-hydroxy Guanosine antibody (15A3) validated for?

This monoclonal antibody is suitable and tested for ELISA, immunohistochemistry (IHC), immunocytochemistry (ICC), and Western blot (WB) applications.

How should the mouse anti-8-hydroxy Guanosine antibody be stored to maintain stability?

The antibody should be stored at temperatures between -20°C and -80°C, and multiple freeze-thaw cycles should be avoided to preserve antibody activity.

What is the immunogen used to generate the mouse anti-8-hydroxy Guanosine (15A3) monoclonal antibody?

The immunogen for this antibody is 8-hydroxy-guanosine, which targets oxidative damage markers in DNA and RNA.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.