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rabbit anti-Ubiquitin polyclonal antibody 5775

$100.00$2,600.00

Antibody summary

  • Rabbit polyclonal to Ubiquitin
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: all
  • Isotype: IgG
  • 100 µL, 25 µL, 1 mL
SKU: 5775parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

serum

applications

ICC/IF, IHC, WB

reactivity

modified proteins

available sizes

1 mL, 100 µL, 25 µL

rabbit anti-Ubiquitin polyclonal antibody 5775

antibody
Database link:
Ubiquitin
Sequence - MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:5000-1:10000 IF/ICC and IHC: 1:500-1:1000
Immunogen
Purified ubiquitin conjugated with glutaraldehyde to KLH
Size and concentration
25, 100, 1000µL and serum
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
serum, 0.04% NaN3 added
Purity
serum
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Ubiquitin plays a fundamental role in cell biology as a key regulator of protein degradation and cellular processes.

More on: cell markers and ubiquitin
Biotechnology
Ubiquitin, a small protein found in nearly all eukaryotic cells, plays a fundamental role in regulating various cellular processes. Its primary function is to mark proteins for degradation through the ubiquitin-proteasome system, a highly controlled and selective process known as ubiquitination. When a protein is destined for degradation or clearance, multiple ubiquitin molecules are covalently attached to specific lysine residues on the target protein, forming a polyubiquitin chain. This ubiquitin chain acts as a signal, directing the tagged protein to the proteasome, a large multi-subunit protein complex responsible for protein degradation. Once delivered to the proteasome, the ubiquitinated protein is unfolded and processed, and its constituent amino acids are recycled for the synthesis of new proteins. Apart from its role in protein degradation, ubiquitin also participates in various non-proteolytic functions, such as DNA repair, endocytosis, and signal transduction, where monoubiquitination or other ubiquitin modifications can serve as regulatory signals. The precise and intricate control of ubiquitin-mediated processes ensures proper cellular homeostasis, protein turnover, and the maintenance of critical cellular functions.

ICC/IF-image-rabbit-anti-Ubiquitin-polyclonal-antibody-5775
Immunofluorescent analysis of HeLa cells stained with rabbit pAb to ubiquitin, 5775, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, 6886, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10µM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions. Note the diffuse cytoplasmic ubiquitin staining in control cells and well defined ubiquitin positive inclusions in the Lc treated cells.
IHC-image-rabbit-anti-Ubiquitin-polyclonal-antibody-5775
Chromogenic immunostaining of a NBF fixed paraffin embedded human hippocampus section from an Alzheimer's Disease case. Rabbit pAb to ubiquitin dilution 1:2,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The 5775 antibody strongly labels the cytoplasm of diseased neurons as identified by their morphology.
WB-image-rabbit-anti-Ubiquitin-polyclonal-antibody-5775
Western blot analysis of HEK293 cell lysates using rabbit pAb to ubiquitin, 5775, dilution 1:5,000 in green and mouse mAb to beta-Tubulin dilution 1:10,000, in red, used as a loading control. [1] protein standard (red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10µM for 16 hours. the lysate was subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8kDa corresponds to monoubiquitin.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

relevant to this product
Western blot
IHC
ICC
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