Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human seminoma stained with anti-SALL4 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

rabbit anti-SALL4 monoclonal antibody (ZR276) 6360

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to SALL4
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Seminoma
Visualization: Nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6360parent Category: antibody Tags: anatomy, SALL4, Urinary Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-SALL4 monoclonal antibody ZR276 6360

antibody
Database link: human O14601
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Seminoma
Immunogen Recombinant fragment (around aa1-185) of human MIC2 protein (exact sequence is proprietary)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Sal-like protein 4 (Zinc finger protein 797) (Zinc finger protein SALL4)
Gene names SALL4,SALL4 ZNF797
Protein family Sal C2H2-type zinc-finger protein family
Mass 112231Da
Tissues TISSUE SPECIFICITY: Expressed in testis. Constitutively expressed in acute myeloid leukemia (AML). {ECO:0000269\|PubMed:16763212}.
Structure SUBUNIT: Interacts with POU5F1/OCT4 (PubMed:23012367). Interacts with NANOG (By similarity). Interacts with BEND3 (PubMed:21914818). Interacts with NSD2 (via PHD-type zinc fingers 1, 2 and 3) (By similarity). Interacts with NRBP1 (PubMed:22510880). {ECO:0000250\|UniProtKB:Q8BX22, ECO:0000269\|PubMed:21914818, ECO:0000269\|PubMed:22510880, ECO:0000269\|PubMed:23012367}.
Post-translational modification PTM: Isoform SALL4B exists primarily as a ubiquitinated form. {ECO:0000269\|PubMed:23012367}.; PTM: Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4. {ECO:0000269\|PubMed:23012367}.
Involvement in disease DISEASE: Duane-radial ray syndrome (DRRS) [MIM:607323]: Disorder characterized by the association of forearm malformations with Duane retraction syndrome. {ECO:0000269\|PubMed:12393809, ECO:0000269\|PubMed:12395297, ECO:0000269\|PubMed:16402211}. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: IVIC syndrome (IVIC) [MIM:147750]: An autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome with a similar phenotype. {ECO:0000269\|PubMed:17256792}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: Q9UJQ4
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human seminoma stained with anti-SALL4 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.