rabbit anti-p53 polyclonal antibody bl2501h11 1023

$100.00 – $300.00

Antibody summary

Rabbit polyclonal to p53
Suitable for: IHC, IP, WB, Flow Cyt
Reacts with: Hu, Ms
Isotype: IgG
100 µL (10 blots), 20 µL

available sizes

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SKU: 1023parent Categories: antibody, oxidative stress Tag: p53

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	monoclonal
concentration	0.1 mg/mL
applications	Flow Cyt, IHC, IP, WB
reactivity	human, mouse
available sizes	100 µL, 20 µL

rabbit anti- p53 polyclonal antibody bl2501h11 1023

antibody
Database link: human P04637 mouse P02340
Tested applications IHC, IP, WB, Flow Cyt
Recommended dilutions Flow Cytometry (Flow Cyt) Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.,Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.,Immunoprecipitation (IP) 20 µl/mg lysate,Wester
Immunogen Between 350 and 400
Size and concentration 100µL and 0.1 mg/mL
Form liquid
Storage Instructions Store at 2-8°C. Expires 1 year from date of receipt.
Storage buffer Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit monoclonal - Isotype Control

target relevance
Protein names Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53)
Gene names TP53,TP53 P53
Mass 43653Da

Data

Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (#1023) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 second.

Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (#1023) used for IP at 20 µl per reaction. 53BP1 was also immunoprecipitated by rabbit anti-53BP1 antibody. For blotting immunoprecipitated 53BP1, #1023 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 second.

Detection of human 53BP1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (#1023) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human 53BP1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (#1023) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (#1023) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of mouse 53BP1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (#1023) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.