| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
rabbit anti-NeuN polyclonal antibody 2615
$100.00 – $2,600.00
Antibody summary
- Rabbit polyclonal to NeuN
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG
- 100 µg, 25 µg, 1 mg
rabbit anti-NeuN polyclonal antibody 2615
| target relevance |
|---|
| NeuN, also known as Fox-3 or RBFOX3, is a well-established neuronal marker widely used in neuroscience research. It is a nuclear protein that is selectively expressed in post-mitotic neurons, making it an essential tool for identifying and characterizing neuronal populations in various tissues and experimental models. NeuN immunohistochemistry, facilitated by specific antibodies targeting NeuN, allows researchers to visualize and quantify neuronal cell bodies, providing valuable insights into neuronal distribution, density, and morphology. This marker is particularly valuable for studying neurodevelopment, neurodegenerative diseases, and neuronal plasticity in response to different experimental conditions or treatments. Moreover, NeuN antibodies have proven instrumental in identifying and differentiating neurons from other cell types, such as glial cells, in complex neural tissues. Click for more on: cell markers and NeuN |
| Protein names RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen) |
| Gene names RBFOX3,RBFOX3 |
| Mass 33873Da |
| Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). |
| Subellular location Nucleus Cytoplasm Note=Largely restricted to neuronal nuclei. However, significant cytoplasmic localization in neurons from brains from HIV-infected individuals with cognitive impairment. |
| Target Relevance information above includes information from UniProt accession : A6NFN3 |
| The UniProt Consortium |
Data
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| Immunofluorescent analysis of a rat brain section stained with rabbit pAb to FOX3/NeuN protein, 2615, dilution 1:2,000 in red, and costained with chicken pAb to microtubule associated protein 2 (MAP2), dilution 1:5,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µm, and free-floating sections were stained with the above antibodies. The FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells, while the MAP2 antibody labels dendrites and overlaps with Fox3/NeuN staining in neuronal perikarya. |
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| Chromogenic immunostaining of a formalin fixed paraffin embedded mouse hippocampus section with rabbit pAb to Fox3/NeuN, 2615, dilution 1:4,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. The 2615 antibody selectively labels the nuclei and distal perikaryal of most neuronal cell populations. This antibody performs well in testing with 4% PFA and NBF fixed mouse, human, and rat tissues. Mouse select image for larger view. |
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| Western blot analysis of cytosolic (cyt) and nuclear enriched (nuc) fractions of whole brain lysates using rabbit pAb to FOX3/NeuN N-terminal peptide, 2615, dilution 1:1,000 in green: [1] protein molecular weight standard (red), [2] rat cyt, [3] rat nuc, [4] mouse cyt, and [5] mouse nuc lysate. Two bands of 46 and 48kDa correspond to the two alternate transcripts of the FOX3/NeuN protein. Western blot was performed under non reducing conditions. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 234 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36312018 | Corrigendum: Age-dependent microstructure alterations in 5xFAD mice by high-resolution diffusion tensor imaging | Maharjan S, Tsai AP, Lin PB, Ingraham C, Jewett MR, Landreth GE, Oblak AL, Wang N. | Front Neurosci. 2022 Oct 13;16:1025457. doi: 10.3389/fnins.2022.1025457. eCollection 2022. |
| 36291186 | Spatial and Temporal Diversity of Astrocyte Phenotypes in Spinocerebellar Ataxia Type 1 Mice | Rosa JG, Hamel K, Sheeler C, Borgenheimer E, Gilliat S, Soles A, Ghannoum FJ, Sbrocco K, Handler HP, Rainwater O, Kang R, Cvetanovic M. | Cells. 2022 Oct 21;11(20):3323. doi: 10.3390/cells11203323. |
| 35972048 | Kctd7 deficiency induces myoclonic seizures associated with Purkinje cell death and microvascular defects | Liang JH, Alevy J, Akhanov V, Seo R, Massey CA, Jiang D, Zhou J, Sillitoe RV, Noebels JL, Samuel MA. | Dis Model Mech. 2022 Sep 1;15(9):dmm049642. doi: 10.1242/dmm.049642. Epub 2022 Sep 13. |
| 35897768 | Reorganization of the Brain Extracellular Matrix in Hippocampal Sclerosis | Sitaš B, Bobić-Rasonja M, Mrak G, Trnski S, Krbot Skorić M, Orešković D, Knezović V, Petelin Gadže Ž, Petanjek Z, Šimić G, Kolenc D, Jovanov Milošević N. | Int J Mol Sci. 2022 Jul 25;23(15):8197. doi: 10.3390/ijms23158197. |
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
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