| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| reactivity | human, mouse, rat |
| available sizes | 1 mg, 100 µg, 25 µg |
rabbit anti-NeuN polyclonal antibody 2615
$100.00 – $2,600.00
Antibody summary
- Rabbit polyclonal to NeuN
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG
- 100 µg, 25 µg, 1 mg
rabbit anti-NeuN polyclonal antibody 2615
| target relevance |
|---|
| NeuN, also known as Fox-3 or RBFOX3, is a well-established neuronal marker widely used in neuroscience research. It is a nuclear protein that is selectively expressed in post-mitotic neurons, making it an essential tool for identifying and characterizing neuronal populations in various tissues and experimental models. NeuN immunohistochemistry, facilitated by specific antibodies targeting NeuN, allows researchers to visualize and quantify neuronal cell bodies, providing valuable insights into neuronal distribution, density, and morphology. This marker is particularly valuable for studying neurodevelopment, neurodegenerative diseases, and neuronal plasticity in response to different experimental conditions or treatments. Moreover, NeuN antibodies have proven instrumental in identifying and differentiating neurons from other cell types, such as glial cells, in complex neural tissues. Click for more on: cell markers and NeuN |
| Protein names RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen) |
| Gene names RBFOX3,RBFOX3 |
| Mass 33873Da |
| Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). |
| Subellular location Nucleus Cytoplasm Note=Largely restricted to neuronal nuclei. However, significant cytoplasmic localization in neurons from brains from HIV-infected individuals with cognitive impairment. |
| Target Relevance information above includes information from UniProt accession: A6NFN3 |
| The UniProt Consortium |
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| Immunofluorescent analysis of a rat brain section stained with rabbit pAb to FOX3/NeuN protein, 2615, dilution 1:2,000 in red, and costained with chicken pAb to microtubule associated protein 2 (MAP2), dilution 1:5,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µm, and free-floating sections were stained with the above antibodies. The FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells, while the MAP2 antibody labels dendrites and overlaps with Fox3/NeuN staining in neuronal perikarya. |
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| Chromogenic immunostaining of a formalin fixed paraffin embedded mouse hippocampus section with rabbit pAb to Fox3/NeuN, 2615, dilution 1:4,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. The 2615 antibody selectively labels the nuclei and distal perikaryal of most neuronal cell populations. This antibody performs well in testing with 4% PFA and NBF fixed mouse, human, and rat tissues. |
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| Western blot analysis of cytosolic (cyt) and nuclear enriched (nuc) fractions of whole brain lysates using rabbit pAb to FOX3/NeuN N-terminal peptide, 2615, dilution 1:1,000 in green: [1] protein molecular weight standard (red), [2] rat cyt, [3] rat nuc, [4] mouse cyt, and [5] mouse nuc lysate. Two bands of 46 and 48kDa correspond to the two alternate transcripts of the FOX3/NeuN protein. Western blot was performed under non reducing conditions. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| 36312018 | Corrigendum: Age-dependent microstructure alterations in 5xFAD mice by high-resolution diffusion tensor imaging. | Surendra Maharjan, Andy P Tsai, Peter B Lin, Cynthia Ingraham, Megan R Jewett, Gary E Landreth, Adrian L Oblak, Nian Wang | Front Neurosci 16:1025457 |
| 36291186 | Spatial and Temporal Diversity of Astrocyte Phenotypes in Spinocerebellar Ataxia Type 1 Mice. | Juao-Guilherme Rosa, Katherine Hamel, Carrie Sheeler, Ella Borgenheimer, Stephen Gilliat, Alyssa Soles, Ferris J Ghannoum, Kaelin Sbrocco, Hillary P Handler, Orion Rainwater, Ryan Kang, Marija Cvetanovic | Cells 11:N/A |
| 35972048 | Kctd7 deficiency induces myoclonic seizures associated with Purkinje cell death and microvascular defects. | Justine H Liang, Jonathan Alevy, Viktor Akhanov, Ryan Seo, Cory A Massey, Danye Jiang, Joy Zhou, Roy V Sillitoe, Jeffrey L Noebels, Melanie A Samuel | Dis Model Mech 15:N/A |
| 35897768 | Reorganization of the Brain Extracellular Matrix in Hippocampal Sclerosis. | Barbara Sitaš, Mihaela Bobić-Rasonja, Goran Mrak, Sara Trnski, Magdalena Krbot Skorić, Darko Orešković, Vinka Knezović, Željka Petelin Gadže, Zdravko Petanjek, Goran Šimić, Danijela Kolenc, Nataša Jovanov Milošević | Int J Mol Sci 23:N/A |
| relevant to this product |
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| Western blot IHC ICC |
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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