| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | chicken |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
rabbit anti-GFAP polyclonal antibody 2539
$100.00 – $2,600.00
Antibody summary
- Rabbit polyclonal to GFAP
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG
- 100 µg
rabbit anti-GFAP polyclonal antibody 2539
| target relevance |
|---|
| Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders. Click for more on: cell markers and GFAP |
| Protein names Glial fibrillary acidic protein (GFAP) |
| Gene names GFAP,GFAP |
| Protein family Intermediate filament family |
| Mass 49880Da |
| Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Subellular location Cytoplasm Note=Associated with intermediate filaments. |
| Tissues Expressed in cells lacking fibronectin. |
| Structure Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus). |
| Post-translational modification Phosphorylated by PKN1. |
| Target Relevance information above includes information from UniProt accession : P14136 |
| The UniProt Consortium |
Data
 |
| Immunofluorescent analysis of a rat cerebellum section stained with rabbit pAb to GFAP, 2539, dilution 1:5,000 in green and costained with mouse mAb to MeCP2 dilution 1:500, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45µM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons. |
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| Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebellum section with rabbit pAb to GFAP, 2539, dilution 1:5,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The 2539 antibody labels the processes of astrocytes within both the granular and molecular layers and Bergmann glia in the molecular layer. This antibody performs well in testing with both 4% PFA and standard NBF fixed rat, mouse, and human tissues. Mouse select image for larger view. |
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| Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with rabbit polyclonal antibody to GFAP, 2539, dilution 1:5,000 in red, and chicken polyclonal antibody to vimentin, 5866, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The astrocytes containing GFAP only are red, GFAP and Vimentin are golden-yellow. Fibroblastic cells contain only vimentin, so they are green. Mouse select image for larger view. |
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| Above is an image generated in the Lifecanvas Technologies laboratory using 2539 and an appropriate secondary antibody on a 1mm section of rat brain. This was processed using the Lifecanvas SHIELD fixation protocol which employs a novel epoxy crosslinker (Park et al. Nature Biotechnology 2018). Mouse select image for larger view. |
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| Western blot analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, 2539, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 1040 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36600259 | Delayed NLRP3 inflammasome inhibition ameliorates subacute stroke progression in mice | Bellut M, Bieber M, Kraft P, Weber ANR, Stoll G, Schuhmann MK. | J Neuroinflammation. 2023 Jan 4;20(1):4. doi: 10.1186/s12974-022-02674-w. |
| 36596518 | Specific Cellular Effects of Low Bortezomib Concentrations on Purified Cultures of Schwann Cells, Satellite Glial Cells, Macrophages, and Dorsal Root Ganglion Neurons | Zhou Z, Nagayasu K, Shirakawa H, Kaneko S. | Biol Pharm Bull. 2023;46(1):102-110. doi: 10.1248/bpb.b22-00668. |
| 36572898 | Lactate attenuates astrocytic inflammation by inhibiting ubiquitination and degradation of NDRG2 under oxygen-glucose deprivation conditions | Xu J, Ji T, Li G, Zhang H, Zheng Y, Li M, Ma J, Li Y, Chi G. | J Neuroinflammation. 2022 Dec 26;19(1):314. doi: 10.1186/s12974-022-02678-6. |
| 36531208 | Inhibition of Prostaglandin E2 Receptor EP3 Attenuates Oxidative Stress and Neuronal Apoptosis Partially by Modulating p38MAPK/FOXO3/Mul1/Mfn2 Pathway after Subarachnoid Hemorrhage in Rats | Liu Y, Liu R, Huang L, Zuo G, Dai J, Gao L, Shi H, Fang Y, Lu Q, Okada T, Wang Z, Hu X, Lenahan C, Tang J, Xiao J, Zhang JH. | Oxid Med Cell Longev. 2022 Dec 9;2022:7727616. doi: 10.1155/2022/7727616. eCollection 2022. |
| 36529767 | The impairment of intramural periarterial drainage in brain after subarachnoid hemorrhage | Sun Y, Liu E, Pei Y, Yao Q, Ma H, Mu Y, Wang Y, Zhang Y, Yang X, Wang X, Xue J, Zhai J, Carare RO, Qin L, Yan J. | Acta Neuropathol Commun. 2022 Dec 18;10(1):187. doi: 10.1186/s40478-022-01492-8. |
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
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| No results found | |||
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